T-DNA插入麝香百合的研究(英文)

• 作 者: 李刚 刘菊华 谭光兰 徐碧玉 金志强
• 机 构: 中国热带农业科学院生物技术研究所,
• 出 处: Agricultural Science & Technology
• 发表日期: 2008年02期
• 关键词: 麝香百合 Lilium DNA transgenic breeding Thumb screening explant preliminarily T-DNA

[Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6-0.8,the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR,preliminarily proving that T-DNA gene had integrated into the genome of lily. [Conclusion] The study may lay a foundation for breeding excellent lily varieties through T-DNA integration technique.

[Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [Result] The best infection The above-mentioned OD600 value of Agrobacterium within 0.6-0.8, the addition of 250 mg / L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg / L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR, preliminarily proving that T-DNA gene had integrated into the genome of lily. [Conclusion] The study may lay a foundation for breeding excellent lily varieties through T-DNA integration technique.