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目的研究三苯氧胺(TAM)对人脑胶质瘤细胞SHG-44增殖的影响并探究其可能机制。方法将不同浓度TAM和脑胶质瘤细胞作用,分别用细胞划痕法、3H-TdR掺入法、流式细胞仪和免疫组化法检测细胞转移能力、细胞DNA合成情况、细胞周期改变和细胞凋亡率及雌激素受体的表达情况。结果TAM在体外能明显抑制SHG-44细胞转移,抑制细胞DNA合成,且抑制作用呈浓度依赖性。流式细胞仪显示细胞经TAM处理后,表现为G0/G1期和G2/M期细胞所占比例增加,而S期细胞所占比例减少。用0、2、10μmol/LTAM处理时细胞凋亡率分别为(2.05±0.28)%、(7.66±0.52)%和(19.00±0.77)%,SHG-44细胞不表达雌激素受体。结论TAM可能通过改变细胞周期和诱导细胞凋亡来抑制脑胶质瘤细胞SHG-44的生长。
Objective To investigate the effect of tamoxifen (TAM) on the proliferation of human glioma SHG-44 cells and to explore its possible mechanism. Methods Different concentrations of TAM and glioma cells were treated by cell scratch method, 3H-TdR incorporation method, flow cytometry and immunohistochemistry to detect the cell migration ability, cell DNA synthesis, cell cycle changes and Apoptosis rate and estrogen receptor expression. Results TAM could inhibit SHG-44 cell migration in vitro and inhibit DNA synthesis in a concentration-dependent manner. Flow cytometry showed that the proportion of cells in G0 / G1 phase and G2 / M phase increased after TAM treatment, while the proportion of S phase cells decreased. The apoptotic rate of SHG-44 cells was (2.05 ± 0.28)%, (7.66 ± 0.52)% and (19.00 ± 0.77)%, respectively. SHG-44 cells did not express estrogen receptor. Conclusion TAM may inhibit the growth of glioma cells SHG-44 by altering the cell cycle and inducing apoptosis.