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目的探讨乳腺癌耐药蛋白在HBV感染胎盘组织中的表达及其意义。方法选取HBV感染足月胎盘20例;选择无HBV感染足月胎盘20例作为正常对照组。实时荧光定量RT-PCR分别检测两组胎盘中ABCG2mRNA的表达。蛋白免疫印迹法分别检测两组胎盘中乳腺癌耐药蛋白的表达。结果各组荧光定量PCR检测ABCG2 mRNA的相对表达量为乙肝组(0.424±0.358),正常组(0.144±0.116),两组相比,差异有显著性(P<0.005)。蛋白免疫印迹法检测乳腺癌耐药蛋白表达量为乙肝组(0.4250±0.3106),正常组(0.1129±0.1045),两组相比,差异有显著性(P<0.001)。结论乳腺癌耐药蛋白在HBV感染胎盘组织中表达的升高,可能与乙型肝炎病毒感染有关。
Objective To investigate the expression of breast cancer resistance protein in HBV infected placenta and its significance. Methods 20 cases of full-term placenta infection by HBV infection were selected. 20 cases of full-term placenta without HBV infection were selected as normal control group. Real-time fluorescent quantitative RT-PCR were used to detect the expression of ABCG2 mRNA in placenta of both groups. Western blotting was used to detect the expression of breast cancer resistance protein in two groups of placenta respectively. Results The relative expression of ABCG2 mRNA in each group was significantly higher than that in hepatitis B group (0.424 ± 0.358) and normal group (0.144 ± 0.116). There was significant difference between the two groups (P <0.005). Western blotting showed that the expression of drug resistance protein in breast cancer was significantly higher than that in hepatitis B group (0.4250 ± 0.3106) and normal group (0.1129 ± 0.1045) (P <0.001). Conclusion The expression of breast cancer resistance protein in HBV infected placenta may be related to hepatitis B virus infection.