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应用室内人工降温,以0.2℃/3 h匀速降温至半数致死温度(8.6℃),然后以0.4、0.7和1.0℃/4h升温至室温。对持续时间、累计昏迷率和累计死亡率进行线性回归分析。结果表明:累计昏迷率与持续时间呈显著的线性相关,0.4℃/4 h上升段和下降段试验组线性回归方程分别为:y=-0.982+0.012x和y=0.929-0.005x;0.7℃/4 h上升段和下降段试验组线性回归方程分别为:y=-1.008+0.012x和y=0.789-0.004x;1.0℃/4 h上升段和下降段试验组线性回归方程分别为:y=-0.460+0.006x和y=0.914-0.006x。累计死亡率与持续时间呈显著的线性相关,0.4、0.7和1.0℃/4 h试验组线性回归方程分别为:y=-0.737+0.008x、y=-0.256+0.004x和y=-0.490+0.005x。累计昏迷率与累计死亡率呈显著的线性相关。0.7和1.0℃/4 h组的回温速度可以作为较理想的回温模式,为后续的耐寒选育提供耐寒的罗非鱼亲本。
Apply indoor artificial cooling to 0.2 ℃ / 3 h uniform temperature to a half lethal temperature (8.6 ℃), and then to 0.4,0.7 and 1.0 ℃ / 4h warmed to room temperature. Linear regression analysis was performed on duration, cumulative coma and cumulative mortality. The results showed that there was a significant linear correlation between cumulative comatose rate and duration. The linear regression equation of the experimental group at 0.4 ℃ / 4 h rising stage and falling stage were: y = -0.982 + 0.012x and y = 0.929-0.005x; 0.7 ℃ The linear regression equations of the experimental group at the rising stage and the descending stage at 4 h respectively were: y = -1.008 + 0.012x and y = 0.789-0.004x; the linear regression equations of the experimental group at the rising stage and the descending stage at 1.0 ℃ / 4 h were: y = -0.460 + 0.006x and y = 0.914-0.006x. There was a significant linear correlation between cumulative mortality and duration. The linear regression equations of the experimental groups at 0.4, 0.7 and 1.0 ℃ / 4 h were: y = -0.737 + 0.008x, y = -0.256 + 0.004x and y = -0.490 + 0.005x. The cumulative coma rate and cumulative mortality rate showed a significant linear correlation. 0.7 and 1.0 ℃ / 4 h group temperature can be used as the ideal temperature mode, providing cold hardy tilapia parents for the subsequent cold hardiness breeding.