论文部分内容阅读
目的探讨急性排斥反应时移植肾组织中程序性死亡配体-1(PD-L1)及程序性死亡-1(PD-1)的表达及其与肾小管间质病理损害程度的关系。方法当肾移植患者发生急性排斥反应并经病理检查确认时,采取移植肾组织,以免疫组化和原位杂交染色法,观察肾组织中PD-L1和PD-1的表达,分析PD-L1阳性强度与肾小管-间质PD-1阳性细胞数、肾小管间质病理损害程度的关系。以正常肾组织为对照。结果急性排斥反应时,移植肾组织中的PD-L1及PD-1的表达较正常组织增多、增强(P<0.01);肾小管PD-L1阳性强度与肾间质PD-1阳性细胞数呈正相关,与肾小管间质病理损害程度呈负相关。结论急性排斥反应时,PD-L1及PD-1的表达增强,它们可能在肾小管间质病理损害中起重要作用。
Objective To investigate the expression of PD-L1 and PD-1 in renal allograft during acute rejection and its relationship with the degree of renal tubulointerstitial lesions. Methods When acute rejection occurred in renal transplant recipients and confirmed by pathological examination, renal allografts were harvested and the expression of PD-L1 and PD-1 in renal tissues was observed by immunohistochemistry and in situ hybridization staining. The relationship between the positive intensity and the number of tubulointerstitial PD-1 positive cells and the degree of tubulointerstitial lesions. Normal kidney tissue as a control. Results The expression of PD-L1 and PD-1 in renal allografts increased compared with normal tissues (P <0.01). The positive expression of PD-L1 in renal tubules was positively correlated with the number of PD-1 positive cells Related to the degree of renal tubulointerstitial lesions was negatively correlated. Conclusions The expressions of PD-L1 and PD-1 are increased in acute rejection, which may play an important role in the pathological damage of tubulointerstitial.