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作者对大白菜(Brassica pekinensis,Rupr)雄性不育两用系可育株与不育株的全生育期进行了有关的生理生化比较分析,结果表明:DNA与RNA的含量在整个生育期两者之间无明显的差异;过氧化物酶及细胞色素氧化物酶同工酶在开花前两者之间没有明显的差异,但开花后,不育株的过氧化物酶同工酶活性明显地高于可育株,而细胞色素氧化酶同工酶酶谱则刚好相反;随着生育期的进行,可育株中蛋白质种类逐渐多于不育株,特别是在开花期表现更为突出,脯氨酸的含量在开花前两者的叶片中无明显的差异,但在开花期,可育株的花苞中含量高于不育株,然而这时在叶片中仍无差异;糖分的含量在初期两者无明显的差异,接近抽薹期直到开花期,不育株中非还原糖的含量低于可育株,而淀粉酶的活性又高于可育株;呼吸强度是开花前可育株高于不育株,开花后则相反;而抗氰呼吸在开花前两者之间无差异,随着生育期的发育,不育株的抗氰呼吸有增高的趋势,但在开花期的花苞中其抗氰呼吸强度急剧下降,而可育株的花苞中的抗氰呼吸强度逐渐升高,结果导致两者之间的差异极显著。
The physiological and biochemical analyzes of the whole growth period of male sterile lines and sterile lines of Chinese cabbage (Brassica pekinensis, Rupr) were carried out. The results showed that the content of DNA and RNA in the whole growth stage There was no significant difference between them; peroxidase and cytochrome oxidase isozymes had no significant difference between the two before flowering, but after flowering, the peroxidase isoenzyme activity of the sterile plants obviously Higher than that of fertile plants, but the pattern of cytochrome oxidase isoenzymes was just the opposite. With the growth period, the protein types of fertile plants gradually more than that of sterile plants, especially in flowering stage, The content of proline had no significant difference between the two leaves before flowering, but at the flowering stage, the content of adventitious buds was higher in the flower buds than in the sterile plants, however, there was no difference in the leaves at this time. The content of sugar At the early stage of flowering, the content of non-reducing sugars in the sterile plants was lower than that in fertile plants and the activity of amylase was higher than that in fertile plants. The respiration rate was the pre-flowering fertile plant Higher than the sterile plants, the opposite after flowering; and anti-cyanide breathing two before flowering There was no difference between them. With the development of the growth period, the cyanidation respiration of the sterile plants tended to increase. However, their resistance to cyanide respiration decreased sharply in the flowering buds. However, Respiration intensity gradually increased, resulting in the difference between the two extremely significant.