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肿瘤促进物TPA(12—0—四癸酰基佛波—13—乙酸酯)是从巴豆中提取的。我国传统医学曾用它作泻药;现有研究证明,TPA是小鼠皮肤肿瘤促进物。本文研究TPA对三株大鼠食管上皮细胞株(RE149、REB_2和REB_2T)的作用,以及这一作用与细胞致瘤能力的关系。三株大鼠食管细胞中的RE149是非致瘤性细胞株,REB_2是前致瘤性细胞株,REB_2T是致瘤性细胞株。本研究比较了这三株细胞对TPA的不同反应性。结果表明,0.1—1200ng/ml范围内的TPA的作用不提高三株细胞的克隆形成率。10ng/ml TPA可诱导RE149细胞经历终末期分化和完全抑制增殖。与此相反,400—800ng/ml的TPA不能明显抑制细胞生长。由此可见,三株细胞对TPA的不同反应性与它们的致瘤能力密切相关。TPA可否用于临床鉴别肿瘤细胞和正常细胞值得进一步研究探讨。
The tumor promoter TPA (12-O-tetradecanoylphorphine 13-acetate) was extracted from Croton. Traditional Chinese medicine has used it as a laxative; existing studies have shown that TPA is a skin tumor promoter in mice. This study investigated the effects of TPA on three rat esophageal epithelial cell lines (RE149, REB_2, and REB_2T) and the relationship between this effect and tumorigenicity. RE149 in three rat esophageal cells is a non-tumorigenic cell line, REB_2 is a pre-tumorigenic cell line, and REB_2T is a tumorigenic cell line. This study compared the different reactivity of these three cells to TPA. The results showed that the effect of TPA in the range of 0.1-1200 ng/ml did not increase the clone formation rate of the three cells. Ten ng/ml TPA can induce RE149 cells to undergo end-stage differentiation and completely inhibit proliferation. In contrast, 400-800 ng/ml of TPA did not significantly inhibit cell growth. It can be seen that the different reactivity of three cells to TPA is closely related to their tumorigenicity. TPA can be used for clinical identification of tumor cells and normal cells worth further study.