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本文报道诱导油棕胚愈伤组织和再生植株的初步结果。当成熟胚在脱分化培养基上培养30—90天时它们逐渐增殖愈伤组织。2,4-D是愈伤组织诱导的重要因素,而2,4-D和NAA结合使用可得更好结果。激动素抑制愈伤组织的形成和生长。实验结果还表明,愈伤组织的诱导率不仅取决于培养基的成份,还取决于供体植物的基因型。在愈伤组织转移到含激动素的培养基上,经过一段时间后分化出具有典型合子胚形态的胚状体。在分化培养基上胚状体可发育为完整植株,有些胚状体经过多次继代培养仍然保留胚状体增殖和植株再生的能力。
This paper reports on the preliminary results of inducing calli embryo callus and regenerating plants. When mature embryos are cultured on dedifferentiation medium for 30-90 days, they gradually proliferate the callus. 2,4-D is an important factor in callus induction, and the combination of 2,4-D and NAA gives better results. Kinetin inhibits callus formation and growth. The experimental results also show that the induction rate of callus depends not only on the composition of the medium but also on the genotype of the donor plant. After the callus is transferred to the kinetin-containing medium, the embryoid body with the typical zygotic morphology is differentiated over time. Embryoid bodies can develop into complete plants on differentiation medium, and some embryoid bodies retain the ability of embryoid body proliferation and plant regeneration after multiple subcultures.