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目的:研究胰岛素样生长因子结合蛋白7(IGFBP-7)在子宫内膜腺癌组织的表达,并分析其与子宫内膜样腺癌临床病理参数之间的关系。方法:选取2011年1月~2012年12月郑州大学第一附属医院病理科存档的手术切除石蜡包埋子宫内膜组织标本共123例,其中子宫内膜样腺癌61例,子宫内膜非典型增生22例,子宫内膜简单性增生10例,正常子宫内膜组织30例。免疫组化SP法检测IGFBP-7的表达,实时荧光定量RT-PCR法检测IGFBP-7 mRNA的表达。分析IGFBP-7的阳性表达率与子宫内膜样腺癌临床病理参数之间的关系。结果:(1)免疫组化法检测显示,子宫内膜样腺癌组织中的IGFBP-7阳性表达率显著高于子宫内膜非典型增生和子宫内膜简单性增生(P<0.05);IGFBP-7的表达与子宫内膜癌的病理分级、超重、合并高血压、合并糖尿病有关(P<0.05);(2)RT-PCR检测结果显示,子宫内膜样腺癌中IGFBP7 mRNA表达量显著高于子宫内膜非典型增生、简单性增生和正常子宫内膜组织(P<0.01)。结论:IGFBP-7基因可能参与了子宫内膜腺癌的发生发展过程。
Objective: To investigate the expression of insulin-like growth factor binding protein 7 (IGFBP-7) in endometrial adenocarcinoma and to analyze its relationship with the clinicopathological parameters of endometrial adenocarcinoma. Methods: From January 2011 to December 2012, the First Affiliated Hospital of Zhengzhou University, pathology archived surgical resection of paraffin-embedded endometrial tissue specimens of 123 cases, of which 61 cases of endometrial adenocarcinoma, endometrial SARS 22 cases of hyperplasia, 10 cases of endometrial simple hyperplasia, 30 cases of normal endometrial tissue. Immunohistochemical SP method was used to detect the expression of IGFBP-7, and IGFBP-7 mRNA was detected by real-time fluorescence quantitative RT-PCR. The relationship between the positive expression rate of IGFBP-7 and clinicopathological parameters of endometrial adenocarcinoma was analyzed. Results: (1) Immunohistochemistry showed that the positive rate of IGFBP-7 in endometrial adenocarcinoma was significantly higher than that in endometrial atypical hyperplasia and endometrial simple hyperplasia (P <0.05); IGFBP (P <0.05). (2) RT-PCR results showed that the expression of IGFBP7 mRNA in endometrial adenocarcinoma was significantly higher than that in endometrial adenocarcinoma Higher than atypical hyperplasia, simple hyperplasia and normal endometrial tissue (P <0.01). Conclusion: The IGFBP-7 gene may be involved in the development of endometrial adenocarcinoma.