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目的探讨葫芦巴籽和桑叶提取混合物(fenugreek seed and mulberry leaf extract,FSML)对胰岛素抵抗糖脂代谢紊乱模型大鼠的降血糖作用及其作用机制。方法建立2个不同的胰岛素抵抗糖脂代谢紊乱大鼠模型,测定大鼠血糖、血脂指标,以及胰岛素受体底物蛋白1(IRS1)、葡萄糖转运蛋白4(GLUT4)、过氧化物酶体增殖物激活受体γ(PPARγ)蛋白的表达。结果对模型1,FSML高、低剂量组均能明显降低模型大鼠的0.5h血糖值及AUC(与模型组比较,P<0.01)。对模型2,FSML高剂量组能显著降低0.5 h血糖值及AUC(与模型组比较,P<0.05)。Western blot实验结果表明,FSML高、低剂量组均能提高GLUT4和PPARγ蛋白的表达(与模型组比较,P<0.05)。结论 FSML具有降血糖作用,其作用机制可能是上调PPARγ蛋白的表达,调节胰岛素信号通路,增加GLUT4蛋白表达,从而增加细胞的葡萄糖摄取,减少外周胰岛素靶组织的胰岛素抵抗。
Objective To investigate the hypoglycemic effect and mechanism of fenugreek seed and mulberry leaf extract (FSML) on insulin resistance and glucose and lipid metabolism in rats. Methods Two different rat models of insulin resistance and glucose and lipid metabolism disorder were established. Blood glucose, blood lipid and insulin receptor substrate protein 1 (IRS1), glucose transporter 4 (GLUT4), peroxisome proliferation Activation of Receptor γ (PPARγ) Protein Expression. Results In Model 1 and FSML high and low dose groups, the 0.5h blood glucose level and AUC of model rats were significantly decreased (P <0.01 compared with model group). For Model 2, FSML high dose group could significantly reduce 0.5 h blood glucose level and AUC (P <0.05 compared with model group). The results of Western blot showed that the expression of GLUT4 and PPARγ protein in both FSML high and low dose groups increased (P <0.05 compared with model group). Conclusion FSML has a hypoglycemic effect. Its mechanism may be upregulation of PPARγ protein expression, regulation of insulin signaling pathway, increase of GLUT4 protein expression, thereby increasing glucose uptake in cells and decreasing insulin resistance in peripheral insulin target tissues.