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目的:探讨胶质细胞源性神经生长因子(GDNF)基因转染培养的视网膜神经细胞的可能性及其表达情况。方法:培养的SD大鼠视网膜神经细胞应用脂质体法转染报道基因(pcDNA3-LacZ)和pcDNA3-GDNF,应用RT-PCR及SDS-PAGE电泳检测其表达情况。结果:pcDNA3-LacZ可转染到视网膜神经细胞。应用RT-PCR可在转染的培养细胞中检测到GDNF转录水平的表达,并可通过SDS-PAGE电泳观察到32KD的表达产物。结论:pcDNA3-LacZ可以作为视网膜神经细胞转基因成功与否的判别指标。pcDNA3-GDNF可转染培养的SD视网膜神经细胞,并可检测到其表达,为青光眼的视功能损害防治提供了一种新的可能。眼科学报1998;14:57—60。
Objective: To investigate the possibility and expression of glial cell line-derived nerve growth factor (GDNF) gene transfected cultured retinal neurons. Methods: The cultured rat retinal neurons were transfected with pcDNA3-LacZ and pcDNA3-GDNF by RT-PCR and SDS-PAGE. Results: pcDNA3-LacZ transfected into retinal nerve cells. The expression of GDNF transcription was detected by RT-PCR in transfected cultured cells, and the expression product of 32KD was observed by SDS-PAGE electrophoresis. Conclusion: pcDNA3-LacZ can be used as a discriminant indicator of the success of transgenic retinal neurons. pcDNA3-GDNF transfected cultured SD retinal neurons and their expression can be detected, which provides a new possibility for the prevention and treatment of optic nerve damage in glaucoma. Ophthalmic Science 1998; 14: 57-60.