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背景与目的:膀胱尿路上皮癌是我国泌尿生殖系统常见的恶性肿瘤。本研究旨在分析中国人膀胱尿路上皮癌中染色体畸变的情况,探讨多色荧光原位杂交(multicolor fluorescence in situ hybridization,M-FISH)技术辅助诊断膀胱尿路上皮癌的可行性和有效性。方法:用随机引物法标记3、7、17号染色体着丝粒及9p21区带探针,对57例膀胱尿路上皮癌间期细胞核进行荧光原位杂交(fluorescence in situ hybridization,FISH),统计染色体畸变情况并分析其与病理分期、分级的关系以及染色体畸变组合诊断膀胱尿路上皮癌的阳性率。结果:3、7、17号染色体和9p21的畸变率分别为47.4%(27/57)、50.9%(29/57)、56.1%(32/57)和59.6%(34/57),畸变与分期无相关性,3、7和17号染色体畸变与病理分级有显著相关性(P<0.01)。四个探针组合诊断膀胱尿路上皮癌的总阳性率为54.4%。结论:M-FISH技术检测有助于探索3、7、17号染色体畸变与病理分级的关系。
Background and Objective: Bladder urothelial carcinoma is a common malignant tumor of our genitourinary system. The purpose of this study was to analyze the chromosomal aberrations in urothelial carcinoma of the urinary bladder in China and to explore the feasibility and effectiveness of multicolor fluorescence in situ hybridization (M-FISH) in the diagnosis of bladder urothelial carcinoma . Methods: The chromosomes of chromosomes 3, 7 and 17 and 9p21 zone probe were labeled with random primers. Fluorescence in situ hybridization (FISH) was performed on 57 cases of urinary bladder cancer with interphase nucleus. Statistical analysis Chromosome aberration and analyze the relationship with the pathological staging, grading and chromosomal aberration combined diagnosis of bladder urothelial carcinoma positive rate. Results: The aberration rates of chromosomes 3, 7, 17 and 9p21 were 47.4% (27/57), 50.9% (29/57), 56.1% (32/57) and 59.6% (34/57) There was no correlation between stage 3 and stage 7 chromosome aberrations and pathological grade (P <0.01). The total positive rate of four probes in diagnosing bladder urothelial carcinoma was 54.4%. Conclusion: The detection of M-FISH is helpful to explore the relationship between chromosome aberration 3,7,17 and pathological grade.