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目的 :探讨精氨酸加压素 (AVP)对大鼠心脏成纤维细胞 (CFs)胶原合成的影响。方法 :采用胰酶消化法分离、培养新生 SD大鼠 CFs,3H-脯氨酸掺入法和羟脯氨酸比色法分别观察不同浓度 AVP及其 V1 受体拮抗剂 [d(CH2 ) 5 Tyr2 (Me) ]AVP对 CFs的影响。结果 :1CFs3H-脯氨酸掺入率随着 AVP浓度的增加而增高 ,其中 10 - 7mol/ L,10 - 6 mol/ L AVP组的 3H-脯氨酸掺入率分别为 (5 41± 96 ) cpm/ 5 0 0 0 cells和 (5 6 5± 72 ) cpm/ 5 0 0 0 cells,较对照组 (16 6± 31) cpm/ 5 0 0 0 cells明显增高 (均 P<0 .0 1) ;10 - 7m ol/ L AVP+ 10 - 7mol/ L[d(CH2 ) 5 Tyr2 (Me) ]AVP组3H -脯氨酸掺入率 (2 6 8± 6 4) cpm/ 5 0 0 0 cells较 10 - 7m ol/ L AVP组明显降低 (P<0 .0 1)。 2 CFs培养上清光吸收值(A值 )随着 AVP浓度的增加而增高 ,其中 10 - 7m ol/ L ,10 - 6 m ol/ L AVP组的培养上清 A值分别为 0 .2 2± 0 .0 1和0 . 2 4± 0 .0 1,明显高于对照组 0 .2 0± 0 .0 1,有统计学意义 (均 P <0 .0 1) ;10 - 7m ol/ L AVP+ 10 - 7mol/ L [d(CH2 ) 5 Tyr2 (Me) ]AVP组的 A值为 0 .19± 0 .0 1,明显低于 10 - 7mol/ L AVP组 (P<0 .0 1)。结论 :AVP促进 SD大鼠 CFs胶原合成 ,其作用可能与 V1 受体介导有关
Objective: To investigate the effect of arginine vasopressin (AVP) on the collagen synthesis of rat cardiac fibroblasts (CFs). Methods: CFs were isolated by trypsin digestion and cultured in neonatal SD rats. 3H-Proline incorporation and hydroxyproline colorimetry were used to observe the effects of different concentrations of AVP and its receptor antagonist [d (CH2) 5 Tyr2 (Me)] AVP on CFs. Results: The incorporation rate of 1CFs3H - proline increased with the increase of AVP concentration. The 3H - proline incorporation rates of 10 - 7mol / L, 10 - 6mol / L AVP group were (5 41 ± 96) ) cpm / 500 cells and (565 ± 72) cpm / 500 cells were significantly higher than those in the control group (16 6 ± 31) cpm / 500 cells (all P <0.01) ); 3H - Proline incorporation rate (2 6 8 ± 6 4) cpm / 500 cells in AVP + 10-7mol / L AVP + 10-7mol / L [d (CH2) 5 Tyr2 (Me) Compared with 10 - 7m ol / L AVP group was significantly lower (P <0.01). The absorbance value (A value) of 2 CFs cultured supernatant increased with the increase of AVP concentration, and the values of A in culture supernatant of 10-7 m ol / L and 10-6 m ol / L AVP group were respectively 0.222 0 .0 1 and 0. 24 ± 0. 0 1, which were significantly higher than that of the control group (0 2 0 ± 0 0 1) (all P <0 01); 10 7 m ol / L The A value of AVP group was significantly lower than that of AVP group (P <0.01) in the group of AVP (P <0.01) . CONCLUSION: AVP can promote collagen synthesis of CFs in SD rats, which may be mediated by V1 receptor