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目的:建立黄芩变异种质——白花黄芩的生根壮苗技术体系,为保存和进一步利用该特殊种质奠定理论与技术基础。方法:以白花黄芩带腋芽的茎段为外植体,在添加不同种类和浓度的植物生长调节剂和附加物的不同基本培养基中进行生根壮苗培养。结果:适宜白花黄芩生根的培养基为1/2 MS(全减半)+IBA 0.02 mg/L+2%蔗糖,根诱导率高达100%;壮苗培养的最适培养基为1/2 MS(全减半)+PP3330.2 mg/L+IBA 0.02 mg/L+2%蔗糖,试管苗绿色,节间正常,生长健壮。结论:1/2 MS(全减半)培养基和较低浓度的蔗糖有利于黄芩试管苗生根;培养基中适当添加IBA能显著提高生根率,根粗壮,根条数多;PP333对白花黄芩试管苗的生长具有明显的矮化作用,适宜浓度能明显改善试管苗的素质,试管苗生长健壮。该试验建立了良好的白花黄芩试管苗的生根壮苗技术。
OBJECTIVE: To establish a root system of Scutellaria baicalensis germplasm, which is a variant of Scutellaria baicalensis Georgi, to lay a theoretical and technical foundation for preservation and further utilization of this special germplasm. Methods: The stems of Radix Scutellariae with axillary buds were used as explants for rooting and seedling culture in different basal medium supplemented with different kinds and concentrations of plant growth regulators and additives. Results: The best rooting medium for Radix Scutellariae was 1/2 MS (all halved) and 0.02 mg / L IBA (2% sucrose), the induction rate was as high as 100% (All halved) + PP3330.2 mg / L + IBA 0.02 mg / L + 2% sucrose, plantlets green, internodes normal, robust growth. Conclusion: 1/2 MS medium and lower concentration of sucrose are beneficial to the rooting of Scutellaria baicalensis in vitro. IBA can significantly increase the rooting rate, The growth of in vitro plantlets had obvious dwarfing effect. Appropriate concentration could obviously improve the quality of in vitro plantlets, and the growth of plantlets was robust. The experiment established a good white Scutellaria tube seedling rooting strong seedling technology.