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目的:观察IL-17mRNA基因在慢性牙周炎和健康牙龈组织中的表达水平变化,探讨IL-17在慢性牙周炎发生发展中的作用。方法:选择慢性牙周炎患者23例,正常对照组15例,记录牙周临床指标,采用Real-time PCR方法定量检测IL-17mRNA在牙龈组织中的表达。结果:慢性牙周炎组IL-17mRNA相对表达量(0.00147±0.00055)显著高于正常牙龈组(0.00047±0.00019)(P<0.01),并且慢性牙周炎组IL-17mRNA表达水平与牙龈指数(r=0.58,P<0.01)、牙周袋探诊深度(r=0.57,P<0.01)、附着丧失水平(r=0.49,P<0.05)呈正相关。结论:Th17相关细胞因子IL-17可能在慢性牙周炎发病机制中发挥致炎作用。
Objective: To investigate the expression of IL-17 mRNA in chronic periodontitis and healthy gingival tissues and to explore the role of IL-17 in the development of chronic periodontitis. Methods: Twenty-three patients with chronic periodontitis and 15 normal controls were enrolled in this study. Periodontal clinical parameters were recorded. Real-time PCR was used to detect the expression of IL-17 mRNA in gingival tissues. Results: The relative expression of IL-17mRNA in chronic periodontitis group (0.00147 ± 0.00055) was significantly higher than that in normal gingival group (0.00047 ± 0.00019) (P <0.01), and the expression of IL-17 mRNA in chronic periodontitis group was significantly lower than that in gingival index r = 0.58, P <0.01). The probing depth (r = 0.57, P <0.01) and attachment loss (r = 0.49, P <0.05) of the periodontal pocket were positively correlated. Conclusion: Th17 related cytokine IL-17 may play an inflammatory role in the pathogenesis of chronic periodontitis.