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为给宁夏优质小麦育种和推广提供依据,本研究以180份宁夏小麦品种(系)为材料,利用分子标记检测脂肪氧化酶(LOX)基因在QLpx.caas-1AL和TaLOX-B1位点的组成情况,分析其分布特点。结果表明,不同等位变异及其组合类型的分布比例不同。在QLpx.caas-1AL位点,除了原有的3种等位变异Xwmc312-227、Xwmc312-235和Xwmc312-247外,还发现了2种新的等位变异。经测序分析,两种新变异条带大小分别为199bp和223bp,暂命名为Xwmc312-199和Xwmc312-223。该位点5种等位变异的分布比例分别为46.11%、32.78%、18.89%、1.11%和1.11%。在TaLOX-B1位点存在2种等位变异,即TaLOX-B1a和TaLOX-B1b,分别占15.56%和84.44%。宁夏小麦LOX基因位点存在8种等位变异组合类型,其中Xwmc312-227/TaLOX-B1b组合类型比例(40.56%)最高,Xwmc312-235/TaLOX-B1b次之(27.78%),Xwmc312-199/TaLOX-B1b和Xwmc 312-223/TaLOX-B1b最低(均为1.11%)。同时,在不同地区和相同地区不同来源品种(系)间,LOX基因的分布也存在差异。总体来看,宁夏小麦低活性LOX基因等位变异类型(Xwmc312-227/TaLOX-B1b)所占的比例明显高于高活性类型(Xwmc312-235/TaLOX-B1a)。
In order to provide a basis for breeding and popularizing high-quality wheat in Ningxia, 180 varieties of Ningxia wheat were used as materials to detect the composition of LOX gene in QLpx.caas-1AL and TaLOX-B1 Situation, analyze its distribution characteristics. The results showed that the distribution of different allelic variations and their combinations were different. At the QLpx.caas-1AL locus, in addition to the three original allelic variants Xwmc312-227, Xwmc312-235 and Xwmc312-247, two new allelic variants were found. After sequencing analysis, the two new variant bands size 199bp and 223bp, temporarily named Xwmc312-199 and Xwmc312-223. The distributions of the five alleles were 46.11%, 32.78%, 18.89%, 1.11% and 1.11% respectively. There are two kinds of allelic variations at TaLOX-B1 loci, TaLOX-B1a and TaLOX-B1b, accounting for 15.56% and 84.44% respectively. There were 8 types of allelic combinations in the LOX gene loci of Ningxia wheat, among which the proportion of Xwmc312-227 / TaLOX-B1b was the highest (40.56%), followed by Xwmc312-235 / TaLOX-B1b (27.78%) and Xwmc312-199 / TaLOX-B1b and Xwmc 312-223 / TaLOX-B1b were the lowest (both 1.11%). At the same time, the distribution of LOX gene was also different in different regions and in different varieties (lines) of the same region. Overall, the proportion of the allelic variant of low activity LOX gene (Xwmc312-227 / TaLOX-B1b) in Ningxia wheat was significantly higher than that of high activity type (Xwmc312-235 / TaLOX-B1a).