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实验用200～250g雄性Fischer 344大鼠,饲养在恒温、恒温和控制光照的(12小时光照,12小时黑暗)环境中。大鼠分两组,实验组饮用水含15%乙醇和5%的蔗糖,对照组饮用自来水,共21天。记录体重、饲料和乙醇的摄入量。对照大鼠喂食采用同实验大鼠对饲法。21天后断头处死,采血后30分钟内用气相色谱法分析乙醇浓度,并即刻取出肝脏和肾脏置于1.15%氯化钾冰冻条件下,称重,绞碎、匀浆和制备微粒体。用雷琐洛菲(resoru-
Experiments 200-250 g male Fischer 344 rats were housed in isothermal, thermostated and controlled lighting (12 hour light, 12 hour dark) environment. Rats were divided into two groups, the experimental group drinking water containing 15% ethanol and 5% sucrose, the control group drinking tap water for a total of 21 days. Record weight, feed and ethanol intake. Control rats were fed with the same experimental rat feeding method. After 21 days, the rats were decapitated. The ethanol concentration was analyzed by gas chromatography within 30 minutes after blood sampling. Immediately removed the liver and kidney were placed in 1.15% potassium chloride frozen condition and weighed, minced, homogenized and microsomes were prepared. With resoru-