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本研究以宫川温州蜜柑(Citrus unshiu Marc.cv.unbergii Nakai)9-10年生成年树和2年生盆栽苗为试材,采用~(14)C示踪,红外线CO_2分析和放射自显影等技术,探讨了宫川温州蜜柑秋梢叶片光合产物输出和输入的模式及其与叶片净光合速率的关系、宫川温州蜜柑光合产物运输受其解剖结构控制的程度以及源—库和秋梢叶片光合同化产物运输的特性。结果表明,秋梢叶片在达到最大叶面积(FLA)10％左右时,叶片已经可以测出光合产物输入,叶片达30％FLA时,输出率达到高峰,在此之后,输入率迅速下降。叶片约达45％FLA时,光合产物的输入率降到零。叶片有一个双向光合产物转运的阶段。光合产物的输出,最早在叶面积达到30.4％FLA时测出。输出率高峰发生在叶片达60％FLA时,随后,叶片继续扩大,但输出率不再提高。叶片的净光合速度随着叶片的增大而提高,净光合作用在叶片约达30％FLA时测出,几乎与叶片的光合产物输出同时启动,当叶片净光合速率不再提高时,叶片的输出也保持一个稳定状态。光合产物运转中,源—库间存在明显的同侧运输现象,同侧运输的强度随源—库的距离而变化。叶片放射自显影图像支持了上述结果,并且提供了一些光合产物输出和输入的特性和直观上的证据。
In this study, Citrus unshiu Marc.cv. Unbergii Nakai 9-year-old annual tree and 2-year-old potted seedlings were used as materials, using ~ (14) C tracing, infrared CO_2 analysis and autoradiography Technology, discussed the pattern of output and input of photosynthate and its relationship with leaf net photosynthetic rate in autumn leaves of Satsuma matsuya in Miyagawa, the degree of the controlled transport of photosynthate of Miyawawa Satsuma mandarin by its anatomical structure, Characteristics of photosynthetic product transport. The results showed that the leaf output of photosynthetic products could be detected at leaf up to about 10% of maximum leaf area (FLA), and reached the peak at 30% FLA. After that, the input rate decreased rapidly. At leaf up to about 45% FLA, the input rate of photosynthate drops to zero. Leaves have a bi-directional photosynthetic product transport phase. The output of photosynthate was detected as early as 30.4% FLA of leaf area. When the output peaked at 60% FLA, the leaf continued to expand, but the output rate no longer increased. The net photosynthetic rate of the leaves increased with the increase of the leaves. The net photosynthesis was detected when the leaf was about 30% FLA, and almost simultaneously with the photosynthate output of the leaves. When the net photosynthetic rate of the leaves no longer increased, The output also maintains a steady state. During the photosynthate operation, there was obvious phenomenon of ipsilateral transport between source and depot, and the intensity of ipsilateral transport changed with the distance of source-depot. Autoradiographic images of the leaves support the above results and provide some evidence of the output and input of photosynthetic products and intuitive evidence.