论文部分内容阅读
本实验通过对11个瑞典家庭2～5年的追踪调查了解变形链球菌(以下简称变链)基因型的变化情况。这11个瑞典家庭包括父亲、母亲、初诊时年龄为3±5/12岁的头胎小孩。从恒牙和乳牙的颊侧龈缘及(牙合)面搜集菌斑,在选择性蔗糖—细菌素液体培养基中进行孵育。然后接种于MBS琼脂平面,经形态和血清型鉴定后,用酚—氯仿法进行DNA抽取,限制性核酸内切酶HaeⅢ进行酶切,并用0.55％琼脂凝胶进行电泳,溴化乙淀染色后在紫外光下观察、
In this study, we tracked the changes of genotypes of Streptococcus mutans (hereinafter referred to as variable strand) through the follow-up survey of 11 Swedish families for 2 to 5 years. The 11 Swedish families include fathers and mothers, first-trimester first-trimester children aged 3 ± 5/12. Plaques were collected from the buccal gingival margin and occlusal surfaces of permanent and primary teeth and incubated in a selective sucrose-bacteriocin liquid medium. Then inoculated on the MBS agar plate, identified by morphology and serotypes, DNA extraction with a phenol-chloroform method, restriction endonuclease Hae III digestion, and 0.55% agarose gel electrophoresis, ethidium bromide staining Under UV light observation,