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【目的】探讨白藜芦醇衍生物白藜芦醇亚甲胺﹙resveratrol methyleneimine,RMI﹚对小鼠酒精性脂肪肝的作用及其作用机制。【方法】通过油红O染色观察RMI对氧化低密度脂蛋白(ox-LDL)诱导的RAW264.7巨噬细胞脂质蓄积的影响;建立小鼠酒精性脂肪肝模型,分别以RMI﹙5 mg/kg和10 mg/kg﹚灌喂6周后,检测酒精性脂肪肝模型小鼠肝脂质、血脂,血清中丙二醛(MDA)、总抗氧化能力(T-AOC)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的含量,以及肝组织中MDA、超氧化物歧化酶﹙SOD﹚和谷胱甘肽过氧化物酶﹙GSH-Px﹚的含量,同时观察肝组织切片病理变化。【结果】RMI可抑制ox-LDL诱导的RAW264.7巨噬细胞脂质蓄积。RMI组小鼠血清中总胆固醇﹙TC﹚、甘油三酯﹙TG﹚、低密度脂蛋白胆固醇﹙LDL-C﹚和肝组织中TC、TG和MDA的含量较模型组显著降低(均P<0.05);而血清中的高密度脂蛋白胆固醇﹙HDL-C﹚、T-AOC,肝组织中SOD、GSH-Px含量较模型组显著升高(P<0.05或P<0.01);肝组织切片观察显示RMI组与模型组比较,肝细胞脂肪变性程度减轻,脂肪粒减少。【结论】RMI可能通过降低脂质过氧化物、增加机体抗氧化能力,而对酒精性脂肪肝有一定的改善作用。
【Objective】 To investigate the effect and mechanism of resveratrol methyleneimine (resveratrol methyleneimine) on alcoholic fatty liver in mice. 【Method】 The effect of RMI on the lipid accumulation of RAW264.7 macrophages induced by ox-LDL was observed by oil red O staining. The model of alcoholic fatty liver in mice was established, and the effects of RMI (5 mg kg, and 10 mg / kg) for 6 weeks. The levels of lipid, lipids, serum MDA, T-AOC and alanine aminotransferase (ALT), aspartate aminotransferase (AST) and the content of MDA, SOD and GSH-Px in the liver tissue and the histopathological changes of liver slices . [Results] RMI could inhibit ox-LDL-induced lipid accumulation in RAW264.7 macrophages. The levels of TC, TG and MDA in serum of RMI group were significantly lower than those of model group (all P <0.05) (P <0.05 or P <0.01). The content of HDL-C, T-AOC and the contents of SOD and GSH-Px in liver tissue in serum were significantly higher than those in model group Compared with the model group, the extent of hepatic steatosis was reduced and the fat particle was reduced. 【Conclusion】 RMI may improve the alcoholic fatty liver by lowering lipid peroxides and increasing body antioxidant capacity.