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目的研究蛋白激酶Cα(PKCα)和细胞间黏附分子-1(ICAM-1)在门静脉高压症患者脾静脉血管的表达,探讨其在门静脉高压症形成机制中的作用。方法对34例门静脉高压症患者的脾静脉与30例对照组织行ICAM-1原位杂交染色。用逆转录-聚合酶链反应(RT-PCR)测定门静脉高压症患者脾静脉血管平滑肌细胞(VSMC)中PKCαmRNA的表达。结果正常脾静脉I-CAM-1原位杂交染色呈阴性或弱阳性,门静脉高压症患者脾静脉呈强阳性,差异有统计学意义(P<0.01)。RT-PCR表明门静脉高压症患者脾静脉VSMC中PKCαmRNA的表达是正常的2.81倍。结论PKCα和ICAM-1过度表达可能是门静脉高压症患者肝外血管结构改变的重要原因;I-CAM-1的激活在门静脉高压症的形成机制中有重要作用。
Objective To investigate the expression of protein kinase Cα (PKCα) and intercellular adhesion molecule-1 (ICAM-1) in the splenic vein of patients with portal hypertension and its role in the pathogenesis of portal hypertension. Methods 34 cases of portal hypertension in patients with splenic vein and 30 cases of control line ICAM-1 in situ hybridization staining. The expression of PKCαmRNA in splenic vein vascular smooth muscle cells (VSMCs) of patients with portal hypertension was detected by reverse transcription polymerase chain reaction (RT-PCR). Results Normal splenic vein I-CAM-1 staining was negative or weakly positive. The splenic vein of patients with portal hypertension was strongly positive, the difference was statistically significant (P <0.01). RT-PCR showed that the expression of PKCαmRNA in splenic vein VSMC in patients with portal hypertension was 2.81 times as normal. Conclusions Overexpression of PKCα and ICAM-1 may be the important cause of extrahepatic vascular structure in patients with portal hypertension. The activation of I-CAM-1 plays an important role in the pathogenesis of portal hypertension.