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目的:观察骨新康对新生大鼠成骨细胞及破骨细胞活性影响。方法:SD大鼠灌胃给药3 d取得含药血清。分离成骨细胞及破骨细胞体外培养,MTT法检细胞活性,AKP检测成骨细胞分化程度,用骨吸收陷窝数量评价破骨细胞活性。结果:与对照组相比,含骨新康血清剂量依赖性地促进成骨细胞增殖;与生理盐水组相比,10%,20%骨新康组含药血清显著促进成骨细胞分泌碱性磷酸酶(P<0.01),抑制骨陷窝的生成。结论:骨新康通过促进成骨细胞的增殖和分化,抑制破骨细胞的活性,对临床骨质疏松的防治具有积极意义。
Objective: To observe the effect of Gu Xin Kang on osteoblasts and osteoclasts in neonatal rats. Methods: SD rats were administered intragastrically for 3 days to obtain serum containing drugs. Osteoblasts and osteoclasts were isolated and cultured in vitro. Cell viability was detected by MTT assay. Osteoblast differentiation was evaluated by AKP. Osteoclast activity was evaluated by the number of bone resorption lacunae. Results: Compared with the control group, the bone-containing xinkang serum could promote the osteoblast proliferation in a dose-dependent manner. Compared with the saline group, 10% and 20% Phosphatase (P <0.01), inhibit the formation of bone lacuna. Conclusion: Gu Xin Kang can promote the proliferation and differentiation of osteoblasts and inhibit the activity of osteoclasts, which has a positive effect on the prevention and treatment of clinical osteoporosis.