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目的探讨人胎盘提取液对高脂血症大鼠的脂蛋白_胆固醇代谢、过氧化作用及血小板聚集功能的影响及其作用机制。方法建立Wistar大鼠高血脂模型,用胎盘提取液0.4ml·(100g·d)-1连续灌胃12d,测定其血清TG、TC、LDL_C、HDL_C及其亚组分HDL 2_C的含量 ;测定全血及肝组织LPO、SOD的活性,并通过脂肪染色观察提取液对肝脏脂肪沉积的影响 ;通过放免法测定血浆中6_酮_PGF1α、TXB 2 含量及血小板最大聚集率。结果胎盘提取液使高脂鼠HDL_C、HDL2_C含量明显升高(P<0.01);TG、TC、LDL_C含量明显降低(P<0.01) ;明显降低高脂鼠血清及肝组织LPO含量(P<0.01),提高SOD活性(P<0.01) ;抑制肝脏脂肪沉积,使脂肪空泡明显减少 ;使血浆中PGI2 含量明显升高(P<0.01),TXB 2 含量无明显变化,并明显降低血小板聚集率(P<0.01)。结论胎盘提取液明显改善高脂大鼠脂蛋白_胆固醇代谢,增强机体抗氧化能力,抑制血小板聚集,并抑制脂肪肝形成,有利于拮抗动脉粥样硬化的发生和发展。
Objective To investigate the effects of human placenta extract on lipoprotein cholesterol metabolism, peroxidation and platelet aggregation in hyperlipidemic rats and its mechanism. Methods The model of hyperlipidemia in Wistar rats was established. The content of TG, TC, LDL_C, HDL_C and its sub-component HDL 2_C in serum was determined by continuous intragastric administration of 0.4ml · (100g · d) -1 placenta extract for 12 days. Blood and liver tissue LPO, SOD activity, and through fat staining observed the extract on liver fat deposition; by plasma radioimmunoassay 6_ ketone _PGF1α, TXB2 content and platelet aggregation rate. Results The contents of HDL_C and HDL_C in placenta extract of rats were significantly increased (P <0.01), the levels of TG, TC and LDL_C were significantly decreased (P <0.01), and the content of LPO in serum and liver of rats were significantly decreased ), And increased SOD activity (P <0.01). Inhibition of hepatic fat deposition significantly reduced fat vacuoles. PGI2 levels in plasma increased significantly (P <0.01), TXB 2 levels did not change significantly, and platelet aggregation rate (P <0.01). Conclusion Placenta extract can significantly improve lipoprotein-cholesterol metabolism, enhance the body’s antioxidant capacity, inhibit platelet aggregation and inhibit the formation of fatty liver, which is beneficial to antagonize the occurrence and development of atherosclerosis.