论文部分内容阅读
目的研究电离辐射诱导小鼠胸腺细胞Ｇ１期阻滞的分子通路。方法ＰＩ染色，流式细胞术检测细胞周期。单克隆抗体免疫荧光标记，流式细胞术检测蛋白表达。结果０５、１．０及２．０ＧｙＸ射线全身照射后１２小时及２．０Ｇｙ照射后２４小时小鼠胸腺细胞Ｇ１期细胞数明显增高。２．０Ｇｙ照射后ｐ５３蛋白表达在照射后１～８小时明显增高；ｐ２１蛋白表达在照射后４～４８小时明显增高；ＭＤＭ２蛋白表达在照射后４小时及８小时明显增高；而ＧＡＤＤ４５蛋白表达未见明显变化。结论０５～２．０ＧｙＸ射线全身照射可诱导小鼠胸腺细胞Ｇ１期阻滞。其分子通路主要是ｐ５３－ｐ２１通路。
Objective To study the molecular pathway of G1 arrest in mouse thymocytes induced by ionizing radiation. Methods PI staining, flow cytometry cell cycle. Monoclonal antibody immunofluorescence labeling, flow cytometry protein expression. Results The number of G1 phase cells in mouse thymocytes was significantly increased at 12 hours after 05, 1.0 and 2.0 Gy X-ray irradiation and at 24 hours after 2.0 Gy irradiation. After 2.0Gy irradiation, the expression of p53 protein was significantly increased from 1 to 8 hours after irradiation; the expression of p21 protein was significantly increased from 4 to 48 hours after irradiation; the expression of MDM2 protein was significantly increased 4 hours and 8 hours after irradiation; while the expression of GADD45 protein See obvious changes. Conclusion 05 ~ 2.0 Gy X-ray can induce G1 phase arrest in mouse thymocytes. Its molecular pathway is mainly p53-p21 pathway.