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目的:观察tumstatin肽对体外培养的视网膜微血管内皮细胞迁移及P38MAPK蛋白表达的影响,初步探讨tumstatin肽抗视网膜内皮细胞迁移的机制。方法:采用细胞划痕实验测定tumstatin肽(T8肽)对血管内皮生长因子(VEGF)诱导下RF/6A细胞(恒河猴视网膜微血管内皮细胞)迁移的影响;Western blotting检测T8肽对VEGF刺激后15,30,45,60min的RF/6A细胞P38MAPK蛋白水平的变化。结果:Tumstatin肽对RF/6A细胞迁移具有抑制作用,且可抑制VEGF对RF/6A细胞的促迁移作用,呈剂量依赖性。正常情况下,RF/6A细胞无P38MAPK蛋白的表达,但VEGF可诱导其表达P38MAPK蛋白,而tumstatin可抑制VEGF诱导的RF/6A细胞P38MAPK蛋白的表达(加入20mg/LT8肽30,45,60min时蛋白表达受到显著抑制,差异有显著性意义,P<0.01)。结论:Tumstatin抑制视网膜微血管内皮细胞的迁移,其作用可能与P38MAPK通路有关。
OBJECTIVE: To observe the effect of tumstatin on the migration of retinal microvascular endothelial cells and the protein expression of P38MAPK in vitro and to explore the mechanism of tumstatin’s anti-retinal endothelial cell migration. Methods: The effect of tumstatin peptide (T8 peptide) on the migration of RF / 6A cells (rhesus monkey retinal microvascular endothelial cells) induced by vascular endothelial growth factor (VEGF) was measured by cell scratch assay. The effect of T8 peptide on VEGF 15, 30, 45, 60min RF / 6A cell P38MAPK protein levels. Results: Tumstatin peptide could inhibit the migration of RF / 6A cells and inhibit the migration of VEGF on the RF / 6A cells in a dose-dependent manner. Normal expression of P38MAPK protein was not found in RF / 6A cells, but VEGF could induce its expression of P38MAPK protein, while tumstatin could inhibit VEGF-induced P38MAPK protein expression in RF / 6A cells (20mg / LT8 peptide at 30, 45 and 60min Protein expression was significantly inhibited, the difference was significant (P <0.01). Conclusion: Tumstatin inhibits the migration of retinal microvascular endothelial cells, which may be related to the P38MAPK pathway.