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利用高碘酸钠(NaIO_4)氧化甘露聚糖(mannan,MN)得到了多醛基氧化甘露聚糖,再以帕米膦酸二钠(Pa)修饰,获得带有双磷酸根的甘露聚糖(Pa-MN).以此产物为基础,利用双磷酸根与钙离子的特异亲和作用通过共沉淀法制备了功能化磷酸钙(CAP)纳米粒,并研究了该纳米粒作为模型抗原鸡卵清蛋白(OVA)佐剂的免疫刺激效果.采用核磁共振、元素分析技术证明甘露聚糖被成功修饰.与伴刀豆球蛋白A(ConA)的特异性结合实验表明,合适的修饰度可保持甘露聚糖结构的完整性.通过动态光散射(DLS)粒度仪、扫描电子显微镜(SEM)、透射电子显微镜(TEM)表征发现,该功能化纳米粒平均粒径为130.4±1.9 nm,且分散性良好.Elisa实验测定血清中抗OVA的IgG水平,结果表明该功能化纳米粒具有良好的免疫刺激效果.
Polydial oxidized mannan was obtained by oxidation of mannan (MN) with sodium periodate (NaIO_4) and then modified with pamidronate (Pa) to obtain mannans with diphosphate (Pa-MN) .According to this product, functionalized calcium phosphate (CAP) nanoparticles were prepared by coprecipitation method using the specific affinity between bisphosphonate and calcium ion, and the nanoparticles as a model antigen chicken Immuno-stimulation effect of ovalbumin (OVA) adjuvant.Magnanometry and elemental analysis proved that mannan was successfully modified.The specific binding experiments with concanavalin A (ConA) showed that the appropriate degree of modification And maintain the integrity of the mannan structure.The average particle size of the functionalized nanoparticles was 130.4 ± 1.9 nm by dynamic light scattering (DLS) particle size analyzer, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) Good dispersibility.Elisa experiment measured serum anti-OVA IgG levels, the results show that the functionalized nanoparticles have a good immune stimulating effect.