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Purpose:The aim of this study was to evaluate the efficacy of a 17-week, 3-component lifestyle intervention for enhancing health behaviors during the coronavirus disease 2019 (COVID-19) pandemic. Methods:A parallel-group (intervention and control) study was conducted amongst 79 airline pilots over a 17-week period during the COVID-19 pandemic. The intervention group (n=38) received a personalized sleep, dietary, and physical activity (PA) program. The control group (n=41) received no intervention. Outcome measures for sleep, fruit and vegetable intake, PA, and subjective health were measured though an online sur-vey before and after the 17-week period. The changes in outcome measures were used to determine the efficacy of the intervention. Results:Significant main effects for time × group were found for International Physical Activity Questionnaire-walk (p=0.02) and for all other outcome measures (p<0.01). The intervention group significantly improved in sleep duration (p<0.01;d=1.35), Pittsburgh Sleep Quality Index score (p<0.01;d=1.14), moderate-to-vigorous PA (p<0.01;d=1.44), fruit and vegetable intake (p<0.01;d=2.09), Short Form 12v2 physical score (p < 0.01; d=1.52), and Short Form 12v2 mental score (p < 0.01; d=2.09). The control group showed significant negative change for sleep duration, Pittsburgh Sleep Quality Index score, and Short Form 12v2 mental score (p<0.01). Conclusion:Results provide preliminary evidence that a 3-component healthy sleep, eating, and PA intervention elicit improvements in health behaviors and perceived subjective health in pilots and may improve quality of life during an unprecedented global pandemic.