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为了研究抗麻疹体液和细胞免疫力的动力学,并对相关的免疫抑制情况进行检测,作者对124名12月龄儿童在常规麻疹-腮腺炎-风疹(MMR)疫苗接种前以及接种后14、22、30和38天进行了调查.采静脉血分离外 周血单核细胞(PBMC)以检测细胞介导免疫功能,分离血浆检测抗体.实验表明,免疫后22和30天之间麻疹病毒血凝素(HA)引起的母细胞转化和麻疹蚀斑减少中和(PRN)抗体滴度曲线非常相似,具有相关性.MMR疫苗免疫后用植物血凝素(PHA)刺激PBMC使淋巴因子发生改变;γ干扰素(IFN-γ)在免疫后14和38天升高,白细胞介素4(IL-4)在14天下降,然后恢复到免疫前水平,IL-10和可溶性IL-2受体(SIL-2r)不发生改变.配对分析表明,CD8+细胞在免疫后14、30、38天增加,自然杀伤(NK)细胞在14和30天增加,CD4+细胞在免疫后22和38天减少.
In order to study the kinetics of anti-measles humoral and cellular immunity, and to examine the associated immunosuppression, we conducted a study of 124 children aged 12 months before and after vaccination with conventional measles-mumps-rubella (MMR) 22, 30 and 38 days were investigated.Cell peripheral blood mononuclear cells (PBMCs) were collected by venous blood to detect cell-mediated immune function and plasma antibody was isolated.Experimental results showed that measles virus hemagglutination between 22 and 30 days after immunization The results showed that there was a very similar and significant correlation between the transformation of blasts and the measles plaque reduction and neutralization (PRN) antibody titer induced by HA, and the changes of lymphokines in PBMC stimulated by phytohemagglutinin (PHA) after MMR vaccine immunization. Interleukin-4 (IFN-γ) increased at 14 and 38 days post-immunization, IL-4 decreased at 14 days and then returned to pre-immune levels, with IL-10 and soluble IL-2 receptor SIL-2r) did not change.The paired analysis showed that CD8 + cells increased at 14, 30, 38 days after immunization, natural killer (NK) cells increased at 14 and 30 days and CD4 + cells decreased at 22 and 38 days after immunization.