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目的 检测卵巢癌 Ｙ Ｎ Ｚ２２ 位点有无杂合性丢失以及与临床病理的关系。方法 采用 Ｐ Ｃ Ｒ 技术、聚丙烯酰胺凝胶电泳银染法，对３０ 例卵巢癌及癌旁组织 Ｙ Ｎ Ｚ２２ 位点扩增片段长度多态性进行分析。结果 以癌旁组织提供的信息计算 Ｙ Ｎ Ｚ２２ 基因频率、杂合度，３０ 例卵巢癌中有信息个体 ２３ 例，杂合度 ７６．７％ ，其中 ３ 例观察到 Ｌ Ｏ Ｈ，频率为１３．０％ 。结论 卵巢癌组织中存在 Ｙ Ｎ Ｚ２２ 位点 Ｌ Ｏ Ｈ 现象，且与肿瘤组织分化程度及临床期别有关。
Objective To detect the loss of heterozygosity and its relationship with clinical pathology in ovarian cancer Y N Z22 locus. Methods PCR amplification of P C R and polyacrylamide gel electrophoresis were used to analyze the length polymorphism of Y N Z22 locus in 30 cases of ovarian cancer and its adjacent tissues. Results The frequency and heterozygosity of Y N Z22 gene were calculated according to the information provided by paracancerous tissues. Twenty-three of the 30 informative individuals with ovarian cancer had a heterozygosity of 76.7%. LOH was observed in 3 of 13 ovarian cancers %. Conclusions Ovarian cancer has the phenomenon of L O H at Y N Z22 locus, which is related to the degree of tumor differentiation and clinical stage.