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目的:探讨miR-567在胰腺癌细胞中的表达及其作用。方法:采用qRT-PCR检测正常胰腺导管上皮细胞系HPDE6-C7及胰腺癌细胞系Panc-1、AsPC-1、HPAC、BxPC-3中miR-567表达。Panc-1细胞转染miR-567过表达慢病毒载体后,分别用CCK-8法、流式细胞术、划痕愈合实验、qRT-PCR、Western blot法检测细胞增殖、凋亡及迁移能力,以及KPNA4 mRNA与蛋白的表达、凋亡相关蛋白表达的变化。结果:miR-567在胰腺癌细胞系Panc-1、AsPC-1、HPAC、BxPC-3中的表达水平均明显低于正常胰腺导管上皮细胞系HPDE6-C7(均P<0.05);miR-567慢病毒转染Panc-1细胞后,增殖能力明显减弱,凋亡率明显增加,划痕愈合率明显降低、KPNA4 mRNA与蛋白表达明显下调、而caspase-3及Bax蛋白表达明显上调(均P<0.05)。结论:miR-567在胰腺癌细胞中表达降低,升高其表达可抑制胰腺癌细胞的生长与迁移能力,其机制可能与下调KPNA4并上调凋亡相关蛋白表达有关。
Objective: To investigate the expression and its role of miR-567 in pancreatic cancer cells. Methods: The expression of miR-567 in normal pancreatic ductal epithelial cell lines HPDE6-C7 and pancreatic cancer cell lines Panc-1, AsPC-1, HPAC and BxPC-3 was detected by qRT-PCR. After transfection with miR-567 lentiviral vector, the cell proliferation, apoptosis and migration ability of Panc-1 cells were detected by CCK-8, flow cytometry, wound healing assay, qRT- As well as KPNA4 mRNA and protein expression, apoptosis-related protein expression changes. Results: The expression levels of miR-567 in pancreatic cancer cell lines Panc-1, AsPC-1, HPAC and BxPC-3 were significantly lower than those in normal pancreatic ductal epithelial cells HPDE6-C7 (all P <0.05) The expression of KPNA4 mRNA and protein was significantly down-regulated while the expression of caspase-3 and Bax was significantly up-regulated in Panc-1 cells after transfection with lentivirus (P < 0.05). CONCLUSION: miR-567 is down-regulated in pancreatic cancer cells and increased its expression inhibits the growth and migration of pancreatic cancer cells. The possible mechanism may be related to down-regulation of KPNA4 and up-regulation of apoptosis-related proteins.