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目的 确立视网膜下腔是否具有支持针对视网膜可溶性抗原 (S抗原 )刺激诱导偏离式免疫反应的能力。方法 将视网膜 S抗原接种于 Wistar大鼠的眼前房和视网膜下腔。抗原接种后 7d,使用 S抗原和完全福氏佐剂免疫受主动物。然后 ,通过足部刺激评估迟发型超敏反应 (DTH)。结果 前房和视网膜下腔注射 S抗原的动物没有发生抗原特异性 DTH。与此相反 ,而未预先进行前房和视网膜下腔抗原接种组动物和结膜下 S抗原接种组动物则诱发了剧烈的 DTH。结论 视网膜下腔针对可溶性抗原具有诱导免疫偏离的能力。
Objectives To establish whether the subretinal space has the ability to support the induction of a deviated immune response against the stimulation of retinal soluble antigen (S antigen). Methods Retinal S antigen was inoculated into the anterior chamber and subretinal space of Wistar rats. Seven days after antigen inoculation, recipients were immunized with S antigen and complete Freund’s adjuvant. Delayed hypersensitivity (DTH) was then assessed by foot stimulation. Results Anterior chamber and subretinal injection of S antigen in animals did not occur antigen-specific DTH. In contrast, animals without hypothyroidism and subconjunctival S antigen inoculation group animals developed vigorous DTH beforehand. Conclusion The subretinal space has the ability to induce immune deviation for soluble antigens.