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目的:研究广藿香油对感染后肠易激综合征(PI-IBS)大鼠肠黏膜机械屏障和免疫屏障的保护作用。方法:将SD大鼠随机分为正常组,PI-IBS模型组、藿香正气液组、广藿香油低、中、高剂量组,每组8只。采用结肠灌注乙酸的方法建立PIIBS大鼠模型。正常组和模型组ig生理盐水,藿香正气液组ig藿香正气液3.3 m L·kg~(-1),广藿香油低、中、高剂量组分别ig广藿香油2,3,4 g·kg~(-1),每日1次,共5 d。采用透射电镜观察结肠黏膜上皮细胞超微结构;采用酶联免疫吸附测定(ELISA)的方法检测各组大鼠血清二胺氧化酶(DAO)和血清免疫球蛋白A(SIg A)含量;采用免疫组化检测结肠肠黏膜细胞间黏附分子-1(ICAM-1)的表达。结果:PI-IBS大鼠结肠上皮细胞微绒毛稀疏,长短不一,有微绒毛断裂现象。广藿香油高剂量组对受损的上皮细胞有改善作用。PI-IBS组血清DAO含量高于正常组(P<0.05)。广藿香油高剂量组血清DAO含量低于PI-IBS组(P<0.05),与正常组比较,无显著性差异。PI-IBS组血清SIg A含量低于正常组(P<0.01)。藿香正气液组、广藿香油高剂量组血清SIg A含量与正常组之间无显著性差异。PI-IBS组ICAM-1蛋白表达IA值明显高于正常组(P<0.01)。广藿香油高剂量组ICAM-1蛋白表达IA值均明显低于模型组(P<0.05),与正常组比较,无显著性差异。结论:广藿香油通过修复受损的肠上皮细胞的超微结构,降低肠道通透性,保护肠黏膜机械屏障。通过促进SIg A分泌,抑制ICAM-1的表达,发挥对PI-IBS免疫屏障的调节作用。
Objective: To study the protective effect of patchouli oil on the mechanical barrier and immune barrier of intestinal mucosa in rats with irritable bowel syndrome (PI-IBS) after infection. Methods: SD rats were randomly divided into normal group, PI-IBS model group, Huoxiangzhengqi Liquid group and patchouli oil low, medium and high dose group, 8 rats in each group. The model of PIIBS rat was established by colonic perfusion of acetic acid. Normal group and model group ig physiological saline, Huoxiangzhengqi liquid ig Huoxiang Zhengqi liquid 3.3 m L · kg ~ (-1), patchouli oil low, medium and high dose groups were patchouli oil 2,3,4 g · kg -1, once daily for 5 days. The ultrastructures of colonic mucosal epithelial cells were observed by transmission electron microscopy. The contents of serum diamine oxidase (DAO) and serum immunoglobulin A (SIg A) were detected by enzyme linked immunosorbent assay (ELISA) The expression of intercellular adhesion molecule-1 (ICAM-1) in colonic mucosa was detected by histochemistry. Results: The microvilli in the colon epithelial cells of PI-IBS rats were sparse and varied in length with microvilli rupture. Patchouli oil high dose group on the damaged epithelial cells have an improving effect. The level of serum DAO in PI-IBS group was higher than that in normal group (P <0.05). The level of DAO in the high-dose patchouli oil group was lower than that in the PI-IBS group (P <0.05), but no significant difference compared with the normal group. Serum SIg A level in PI-IBS group was lower than that in normal group (P <0.01). Huoxiangzhengqi liquid group, high dose of patchouli oil serum SIg A content and no significant difference between the normal group. The IA value of ICAM-1 protein expression in PI-IBS group was significantly higher than that in normal group (P <0.01). The IAA of ICAM-1 protein in the high-dose patchouli oil group was significantly lower than that in the model group (P <0.05), but no significant difference compared with the normal group. Conclusion: Patchouli oil can repair intestinal epithelial ultrastructure, reduce intestinal permeability and protect the mechanical barrier of intestinal mucosa. By promoting the secretion of SIg A and inhibiting the expression of ICAM-1, it can regulate the immune barrier of PI-IBS.