论文部分内容阅读
目的 观察开口箭皂苷对S-180肉瘤细胞荷瘤小鼠NF-κB mRNA的影响,证实开口箭对实体瘤的抑制作用,探讨其分子机制.方法 计算四噻唑蓝(MTT)法检测不同浓度的开口箭皂苷对S-180肉瘤细胞体外培养下抑制率,并进行电镜形态观察.同时建立S-180肉瘤荷瘤小鼠模型,给予不同浓度的开口箭皂苷进行干预,以5-氟尿嘧啶(5-Fu)为阳性对照,观察细胞形态,用凝胶电泳迁移率实验(EMSA)技术检测NF-κB的变化;实时荧光定量PCR技术(FQ-PCR)检测各组NF-κB mRNA.结果 开口箭皂苷呈浓度依赖性抑制S-180肉瘤细胞增殖,以48h作用最佳.开口箭各组荷瘤裸鼠的瘤质量呈浓度依赖性下降,差异均有统计学意义(均P＜0.05),各给药组瘤组织NF-κB p65 mRNA及蛋白表达水平均明显低于空白组(均P＜0.05),并随着开口箭皂苷浓度的增加逐渐降低,各组间比较差异均有统计学意义(均P＜0.05).结论 开口箭皂苷明显抑制S-180肉瘤细胞体外增值,具有抑制实体肉瘤作用,降低NF-κB mRNA是其分子机制之一.“,”Objective To study the effect of saponins from Tupistra chinensis Bak (STCB) on the growth of sarcoma S-180 cells in vitro and in mouse xenografts as well as the underlying mechanisms.Methods Cell proliferation was assessed by MTT assay.Cell cycle distribution was determined by flow cytometry.Sarcoma S-180 tumor-bearing mice were treated with different doses of STCB with 5-fluorouracil (5-Fu) as a positive control.The activity of nuclear factor (NF)-κB was detected by gel mobility shift assay.The mRNA level of NF-κB was determined by real-time quantitative RT-PCR.Results In vitro STCB inhibited the growth of S-180 cells in a concentration-dependent manner,which was accompanied by cell cycle arrest at S-phase.In vivo STCB significantly inhibited the growth of S-180 tumor mouse xenografts in a dose-dependent manner with apparent induction of cell apoptosis.Moreover,STCB inhibited the activity of NF-κB p65 and reduced the expression of NF-κB p65 mRNA in mouse xenografts.Conclusions STCB inhibits the proliferation and cell cycle progression of S-180 cells by suppressing NF-κB signaling in mouse xenografts.Our findings suggest STCB is a promising agent for the treatment of sarcoma.