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在标记脐血造血祖细胞表面抗原（ＨＰＣＡ），ＣＤ３４中，比较抗－ＨＰＣＡ－２－ＦＩＴＣ和Ｔｋ３（纯抗体）标记的ＣＤ（３４＋）细胞在流式细胞仪分析中的荧光特征及两种单抗标记的ＣＤ（３４＋）细胞与体外培养的粒单细胞集落形成单位（ＣＦＵ－ＧＭ），红系爆发形成单位（ＢＦＵ－Ｅ），和混合集落形成单位（ＣＦＵ－Ｍｉｘ）的相关性。结果发现脐血有核细胞中，抗ＨＰＣＡ－２阳性细胞占１．０５±０．７２％（ｎ＝１３），Ｔｋ３阳性细胞占２．０６±１．２５％（ｎ＝８），差别显著（Ｐ＜０．０５）。每毫升脐血两种抗体标记的细胞分别为９６．５６±５６．６４和２３１．４０±１６３．９３（Ｐ＜０．０５）。尽管ＨＰＣＡ－２阳性细胞与Ｔｋ３阳性细胞数量呈显著正相关（ｒ＝０．８７５，Ｐ＜０．０１），前者与ＣＦＵ－ＧＭ，ＢＦＵＥ，ＣＦＵ－Ｍｉｘ及集落总数ＣＦＵｓ均呈正相关，而后者仅与ＣＦＵ－ＧＭ，ＣＦＵｓ相关。研究提示在检测造血祖细胞时，用抗－ＨＰＣＡ－２－ＦＩＴＣ代替Ｔｋ３可降低假阳性，获得较好的ＯＤ（３４＋）细胞与ＣＦＵ间的线性关系。
Fluorescence characteristics in flow cytometric analysis of CD34 (34+) cells labeled with anti-HPCA-2-FITC and Tk3 (pure antibody) were compared in labeled cord blood hematopoietic progenitor cell surface antigen (HPCA) The correlation between monoclonal antibody labeled CD34 (34+) cells and cultured monocyte colony forming units (CFU-GM), erythropoietic burst forming units (BFU-E), and mixed colony forming units (CFU-Mix) The results showed that HPCA-2 positive cells accounted for 1.05 ± 0.72% (n = 13), Tk3 positive cells accounted for 2.06 ± 1.25% (n = 8) in umbilical cord blood cells, the difference was significant (P <0.05). Cells labeled with two antibodies per ml of cord blood were 96.56 ± 56.64 and 231.40 ± 163.93, respectively (P <0.05). Although there was a positive correlation between HPCA-2 positive cells and Tk3 positive cells (r = 0.875, P <0.01), the former was positively correlated with CFU-GM, BFUE, CFU- Only related to CFU-GM and CFUs. The study suggests that the anti-HPCA-2-FITC instead of Tk3 can reduce the false positive rate and obtain a good linear relationship between OD (34+) cells and CFU when detecting hematopoietic progenitor cells.