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目的建了高效液相色谱法同时测定果蔬中的叶黄素(Lut)、玉米黄素(Zea)、β-隐黄素(Cryp)、α-胡萝卜素(α-C)和β-胡萝卜素(β-C)的水平。方法均质后的样品经皂化、石油醚提取、水洗和浓缩后,以含2%水的甲醇-乙腈混合液(25:75)和叔丁基甲基醚为流动相,在Waters C30柱中分离,二极管阵列检测器检测,外标法定量。结果与结论上述5种胡萝卜素在0.05～10 g/ml范围内有良好的线性,相关系数r均大于0.997,三水平加标回收率均大于80%(α-C除外),每个加标水平的6次重复试验RSD<12.7%。该方法同时测定了蔬菜中的Lut、Zea、Cryp、α-C、β-C等5种类胡萝卜组分的含量,方法简便、快速、准确。用本法对市场春季常见蔬菜和水果进行了测定,测定结果与文献报道结果基本相符。
Objective To establish a method for the simultaneous determination of Lut, Zea, Cryp, α-C and β-carotene in fruits and vegetables by high performance liquid chromatography (β-C) level. After homogenized, the sample was saponified, extracted with petroleum ether, washed with water and concentrated. The sample was separated on a Waters C30 column with methanol-acetonitrile mixture (25:75) and tert-butyl methyl ether (2% Diode array detector detection, external standard quantitative. RESULTS AND CONCLUSION The above five kinds of carotenoids showed a good linearity in the range of 0.05-10 g / ml with the correlation coefficients r greater than 0.997 and the recoveries of the three levels exceeding 80% (except α-C). Each spiked The level of six replicate trials RSD <12.7%. The method simultaneously determined the contents of five kinds of carrot components of Lut, Zea, Cryp, α-C and β-C in vegetables, and the method is simple, rapid and accurate. This method was used to determine the common vegetables and fruits in the market in spring. The results of the determination are in good agreement with those reported in the literature.