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通过农杆菌(Agrobacterium tumefaciens)致瘤,从大豆属三个种的蛋白质含量不同的627份种质资源中,筛选出致瘤材料223份。其中蛋白质含量在43.00—45.52％的栽培大豆(Glycine max)品种4份;蛋白质含量在44.00—49.55％的半野生大豆(Glycine gracilis)类型23份;蛋白质含量在48.00—51.79％的野生大豆(Glycine soja)类型19份。通过组织培养,从瘤组织中得到了脱菌的愈伤组织。生化检测表明,3个种的大豆瘤来源的部分愈伤组织含有T—DNA。并通过液体培养,建立了含T—DNA的细胞系。现已培养50多代,胭脂碱合成酶基因仍然稳定地整合在大豆基因组中,其染色体数为2n=40,表明是含T—DNA的稳定的细胞系。为基因转移打下了良好的基础。 T_1质粒作为植物基因工程的载体受到了广泛的重视,它的载体功能是通过擦伤感染致瘤的过程进入植物细胞来实现的,因此,通过致瘤反应筛选出理想的受体显得非常重要。我们对大豆属的致瘤反应曾作过一些报道,为了充分利用我国大豆的丰富资源,选育出高蛋白品种,我们进行了基因转移的高蛋白受体系统的研究,本文报道这些研究的初步结果。
Tumorigenicity was induced by Agrobacterium tumefaciens, and 223 of the tumorigenic materials were screened from 627 germplasm resources with different protein content in three species of Glycine. Among them, 4 cultivars of soybean (Glycine max) with protein content of 43.00-45.52%, 23 strains of semi-wild soybean (Glycine gracilis) with protein content of 44.00-49.55%, Glycine max of 48.00-51.79% soja) type 19 copies. Decellularized callus is obtained from the tumor tissue by tissue culture. Biochemical tests showed that some of the three kinds of soybean tumor-derived calluses contained T-DNA. And through liquid culture, a T-DNA-containing cell line was established. Now that it has been cultured for more than 50 generations, the nopaline synthase gene is still stably integrated in the soybean genome with a chromosome number of 2n = 40, indicating that it is a stable cell line containing T-DNA. Gene transfer has laid a good foundation. T_1 plasmid as a plant genetic engineering vector has received extensive attention, and its carrier function is through the process of scratching the tumorigenic tumor cells into the plant to achieve, therefore, through tumorigenic reaction screening of the ideal receptor is very important. We made some reports on the tumorigenic reaction of soybean genus. In order to make full use of the abundant resources of soybean in China and breed high protein varieties, we carried out the research of high protein receptor system of gene transfer. result.