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青蒿素是从菊科植物黄花蒿(Artemisia annua L)中分离提取的一种倍半萜化合物。它对红内期疟原虫具有良好的抗疟活性。本文旨在观察青蒿素在体外试验系统中的抗疟作用及其对疟原虫超微结构的影响。海南株红内期恶性疟用Trager and Jensen的蜡烛缸方法连续体外培养,所用的培养基为RPM I1640。结果表明,在体外试验中,青蒿素可以明显地妨害恶性疟无性体的生长裂殖,其有效浓度为10~(-7)～10~(-6)M。这提示青蒿素对体外培养的恶性疟无性体有直接的杀伤作用。在10~(-7)M或5×10~(-7)M青蒿素作用下,恶性疟无性体的超微结构在药后2小时或1小时便开始出现明显的病理变化,其主要有两方面:(1)膜系结构的损伤。
Artemisinin is a sesquiterpenoid compound isolated from the Compositae artemisia annua L. It has good anti-malarial activity against red-phase Plasmodium. This article aims to investigate the anti-malarial effect of artemisinin in an in vitro test system and its effect on the ultrastructure of Plasmodium. Hainan red-phase falciparum malaria by Trager and Jensen candle cylinder continuous culture in vitro, the medium used for the RPM I1640. The results showed that artemisinin could obviously inhibit the growth and fission of P. falciparum in vitro, and its effective concentration was 10 -7 -7 10 -6 M. This suggests that artemisinin has a direct killing effect on P. falciparum in vitro. Under 10 ~ (-7) M or 5 × 10 ~ (-7) M artemisinin, the ultrastructure of the falciparum malaria ameloblasts began to show obvious pathological changes at 2 hours or 1 hour after drug treatment, and its main There are two aspects: (1) membrane structure damage.