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以黄将甘蓝试管苗幼叶为材料分离原生质体，用ＫＭ８Ｐ培养基进行液体浅层培养和薄层漂浮培养。原生质体的培养密度为６×１０３～１０４／ｍｌ。在薄层漂浮培养中，２天后开始第一次分裂；第５天和７天的分裂频率分别达２３％和３０％。培养１０天后加入稀释培养液，并从暗培养转至弱光照条件下培养，以后每周加一次稀释液。培养１５天后形成小细胞团；３０～３５天形成肉眼可见的小愈伤组织，植板率为３．５％。愈伤组织在含ＩＢＡ０．５～１．０ｍｇ／Ｌ，６—ＢＡ１．５～５．０ｍｇ／Ｌ的分化培养基上，培养约１个月可获得再生无根苗，分化频率可达６８．５％。再生小苗转入合ＮＡＡ０．２ｍｇ／Ｌ的生根培养基，可获得具根的完整再生小植株。
Protoplasts were isolated from the young leaves of Brassica campestris plantlets with KM8P medium for shallow culture and thin-layer culture. Protoplast culture density of 6 × 103 ~ 104 / ml. In lamellar floating culture, the first division started after 2 days; the division frequencies on day 5 and 7 were 23% and 30% respectively. After 10 days of culture, add diluted culture medium, and from dark culture to weak light culture conditions, add a weekly dilution. After 15 days of culture, small clumps formed; 30 to 35 days to form small callus visible to the naked eye, the plating rate was 3.5%. Callus in IBA containing 0.5 ~ 1.0mg / L, 6-BA1.5 ~ 5.0mg / L differentiation medium, cultured for about 1 month to obtain regenerated rootless seedlings, differentiation frequency up to 68.5 %. Regeneration of seedlings into NAA0.2mg / L rooting medium, can be obtained with a complete regeneration of rooted plantlets.