论文部分内容阅读
目的:探究苦参碱联合顺铂对宫颈癌Si Ha细胞株、肺癌肿瘤抑制因子1(TSLC1)基因的表达及Si Ha细胞株对顺铂敏感性的影响,为宫颈癌的临床治疗提供根据。方法:采取四甲基偶氮唑蓝(MTT)法测定不同浓度苦参碱组、顺铂组以及联合组干预Si Ha细胞株48 h后的抑制率;采取实时荧光定量PCR(FQ-PCR)技术检测TSLC1 mRNA表达程度的变化。结果:苦参碱及顺铂对Si Ha细胞株的抑制率均呈剂量依赖关系,随着各组浓度的增高,TSLC 1mRNA的表达上调(P<0.05),不同浓度联合组对Si Ha细胞株的抑制率分别为40.13%、62.88%、67.35%、71.33%和75.11%,TSLC1 mRNA的表达量分别为8.61±0.02、14.29±0.01、27.52±0.06、62.19±0.01和93.12±0.01,与不同浓度苦参碱组及不同浓度顺铂组比较,差异均有统计学意义(P<0.05)。结论:苦参碱联合顺铂可抑制宫颈癌Si Ha细胞株的生成和发展,此机制可能和TSLC1 mRNA的表达上调有关,且苦参碱可以增强Si Ha细胞株对顺铂的敏感性。
Objective: To investigate the effect of matrine and cisplatin on the expression of Si Ha cell line, tumor suppressor 1 (TSLC1) gene in lung cancer and sensitivity of Si Ha cell line to cisplatin, and to provide basis for the clinical treatment of cervical cancer. Methods: MTT assay was used to determine the inhibitory rates of Si ha cell lines treated with different concentrations of matrine, cisplatin and combination groups for 48 h. Real-time fluorescence quantitative PCR (FQ-PCR) To detect the changes of TSLC1 mRNA expression level. Results: The inhibitory rates of matrine and cisplatin on Si Ha cell lines were dose-dependent. The expression of TSLC 1 mRNA was up-regulated with each concentration increasing (P <0.05) The inhibitory rates of TSLC1 mRNA were 40.13%, 62.88%, 67.35%, 71.33% and 75.11%, respectively. The expression of TSLC1 mRNA were 8.61 ± 0.02,14.29 ± 0.01,27.52 ± 0.06,62.19 ± 0.01 and 93.12 ± 0.01, Matrine group and different concentrations of cisplatin group, the difference was statistically significant (P <0.05). Conclusion: Matrine combined with cisplatin can inhibit the generation and development of cervical cancer cell line Si Ha. This mechanism may be related to the upregulation of TSLC1 mRNA, and matrine can enhance the sensitivity of Si Ha cell line to cisplatin.