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目的:探讨青藤碱(SIN)对小鼠皮肤移植排斥反应的免疫抑制作用。方法:BALB/c小鼠作为供体,C57BL/6小鼠作为受体,建立背-背皮肤移植模型。术后分为假手术组、模型组、SIN组(30 mg·kg~(-1))、环孢素A(Cs A)组(10 mg·kg~(-1))、SIN+亚剂量Cs A组(SIN 30 mg·kg~(-1)+Cs A 5 mg·kg~(-1)),每组10只,腹腔注射给药10d。记录各只小鼠移植皮片的存活天数,术后第4天和第8天采用酶联免疫吸附法(ELISA)检测血浆白细胞介素-2(IL-2)含量。结果:与模型组比较,各给药组动物的移植皮片存活时间均有不同程度的延长(P<0.01);SIN与亚剂量Cs A组的小鼠移植皮片存活时间显著长于SIN组或Cs A组(P<0.05或P<0.01);各给药组第4天和第8天的血浆IL-2水平均较模型组明显降低(P<0.01)。结论:SIN具有抑制皮肤移植小鼠免疫排斥反应的作用,与Cs A联合应用可能具有协同作用。
Objective: To investigate the immunosuppressive effect of sinomenine (SIN) on the skin allograft rejection in mice. Methods: BALB / c mice were used as donors and C57BL / 6 mice as recipients to establish a dorsal-dorsal skin graft model. The rats in sham operation group, model group, SIN group (30 mg · kg -1), cyclosporin A (10 mg · kg -1), SIN + sub-dose Cs A group (SIN 30 mg · kg -1 and CsA 5 mg · kg -1), 10 rats in each group were injected intraperitoneally for 10 days. The survival days of the transplanted skin of each mouse were recorded. The levels of plasma interleukin-2 (IL-2) were detected by enzyme-linked immunosorbent assay (ELISA) on the 4th and 8th day after operation. Results: Compared with the model group, the survival time of transplanted skin graft in each group was prolonged (P <0.01). The survival time of transplanted skin graft in SIN and sub-dose CsA group was significantly longer than that in SIN group or CsA group (P <0.05 or P <0.01). The levels of plasma IL-2 on the 4th and 8th day in each treatment group were significantly lower than those in the model group (P <0.01). CONCLUSIONS: SIN can inhibit the immune rejection in skin-grafted mice and may have a synergistic effect with CsA.