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目的 分析上海地区丙型肝炎病毒 (hepatitisCvirus ,HCV)Ⅱ、Ⅲ型包膜区cDNA序列变异。方法 采用逆转录 聚合酶链反应 (RT PCR)扩增HCV包膜区E1、E2 /NS1片断 (10 0 5bp) ,在373DNA全自动测序仪上进行了序列分析。结果 HCV E1、E2 /NS1区核苷酸和氨基酸同源性比较显示 :上海株Ⅱ型、Ⅲ型间同源性分别为 6 2 .48%和 5 9.10 % ;上海株与国内外同型株间分别为85 .97%～ 87.36 %和 83.38%～ 86 .5 7% ;上海株与国内外不同型株间分别为 6 1.49%～ 70 .95 %和5 9 .10 %～ 78.2 1%。此外 ,共发现 5个变异较大的区域 ,其中两个是国际公认的高变区 (HVR1及HVR2 ) ,另外 3个为首次报道 (第 2 5 1～ 2 5 8、2 97～ 30 1、46 1～ 46 7位氨基酸 )。结论 HCV E1、E2 /NS1序列变异大 ,这对其疫苗设计有参考价值
Objective To analyze the variation of cDNA sequence of HCV type Ⅱ and Ⅲ in Shanghai area. Methods E1, E2 / NS1 fragments (105bp) were amplified by reverse transcription polymerase chain reaction (RT PCR) and sequenced on a 373 DNA sequencer. Results The nucleotide and amino acid homology of HCV E1 and E2 / NS1 showed that the homologies of type Ⅱ and type Ⅲ in Shanghai strain were 62.48% and 59.10% respectively. Respectively 85.97% ~ 87.36% and 83.38% ~ 86.57%. The strains were 6 1.49% -70.95% and 5 9.10% -78.2 1% in Shanghai and those in other countries at home and abroad. In addition, a total of five regions with large variation were identified, two of which were internationally recognized hypervariable regions (HVR1 and HVR2) and the other three were first reported (251-158, 277-301, 46 1 to 46 7 amino acids). Conclusion The sequence variation of HCV E1 and E2 / NS1 is large, which has reference value for its vaccine design