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同核体(homokaryon)是进行双孢蘑菇遗传分析和菌种选育的基本材料。我们曾经报道过应用原生质体技术可以在实验室条件下较方便地用菌丝体分离出大量的原生质体同核体(protoplasted homokaryon),这无疑为加快双孢蘑菇遗传育种研究的进程创造了条件。但是由于双孢蘑菇的菌丝一般是多核的,而且又没有锁状联合标记,因此对所获得的原生质体同核体的真实性缺乏一种简单可靠的检测手段,而只能根据它们在再生时间上的先后顺序以及菌落的形态特征进行推定。鉴此,本研究,旨在寻找到可行的方法对推定的原生质体同核体进行有效的验证,结果报道如下: 1 材料与方法 1.1 供试菌株 双孢蘑菇176菌株,上海市农科院食用菌所提供。P_9、P_(11)、P_(12)、P_(14)、P_(15)、P_(20)、P_(21)、P_(22)为随机挑选出的八个推定的176原生质体同核体。 1.2 培养基及配方 ①PDM:马铃薯200g,葡萄糖
Homokaryon (homokaryon) is the basic material for Agaricus bisporus genetic analysis and breeding. We have previously reported that using protoplast technology to isolate large numbers of protoplasted homokaryons from mycelium under laboratory conditions is undoubtedly the key to speeding up the process of genetic breeding of Agaricus bisporus . However, since the mycelium of Agaricus bisporus is generally multinucleated and there are no interlocking markers, there is a lack of a simple and reliable detection method for the authenticity of the obtained protoplasts and nucleosomes, Temporal order and the morphological characteristics of colonies are presumed. In view of this, this study aims to find a viable method for protoplasmic proton nuclear validation validated results are reported as follows: 1 Materials and methods 1.1 test strains Agaricus bisporus 176 strains of Shanghai Academy of Agricultural Sciences Provided by the bacteria. P_9, P_ (11), P_ (12), P_ (14), P_ (15), P_ (20), P_ (21) and P_ (22) are randomly selected eight putative 176 protoplasts with nuclear body. 1.2 medium and recipe ① PDM: potato 200g, glucose