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以温室栽培生长7～8周的烟草（Nicotianatobacum）第4片真叶为材料，测定了脱乙酰几丁质对细胞膜透性的影响。结果发现，脱乙酰几丁质处理的烟草叶片的电解质、可溶性蛋白质的外渗量和胞外可溶性过氧化物酶的活性均比对照组大，并且随所用的脱乙酰几丁质浓度增加而增大。Mg2＋、Ca2＋、Al3＋能抑制脱乙酰几丁质诱导的蛋白质外渗。脱乙酰几丁质（500μgml-1）处理的烟草叶片的几丁醇和β－1，3－葡聚精酶的活力，在处理6h后开始上升，至36h活力达最高峰，分别是对照组的1．88倍和1．51倍。5mmolL-1Ca2＋对这种诱导具有抑制作用。伴随着对几丁酶和β-1，3-葡聚糖酶的诱导，处理组的可溶性蛋白含量也在处理后36h达到最高峰。
The 4th leaf of Nicotianatobacum which was cultivated for 7-8 weeks in greenhouse was used as material to determine the effect of chitosan on cell membrane permeability. As a result, it was found that the chitosan-treated tobacco leaves had larger amounts of electrolyte, extravasation of soluble proteins, and extracellular soluble peroxidase activity than the control group, and increased with the concentration of chitosan used Big. Mg2 +, Ca2 + and Al3 + can inhibit chitosan-induced protein extravasation. The activities of chitosan and β-1,3-glucanase in chitosan treated leaves (500μgml-1) increased after 6 hours and peaked at 36 hours, which were respectively 1.88 times and 1.51 times. 5 mmol L-1 Ca2 + has an inhibitory effect on this induction. With the induction of chitin and β-1, 3-glucanase, the soluble protein content of the treated group also reached the peak at 36 h after treatment.