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以聚合酶链反应（PCR）、聚丙烯酰胺凝胶垂直电泳和银染法对中国100名无关个体小卫星区域p33．4位点的扩增片段长度多态性（Amp－FLPs）进行了研究，检出了8个等位基因。通过BIOTRAC系统进行数据处理．各等位基因重复单位的数目分别为7、10到15，其中在13～14之间发现一差值不足一个重复单位长度的罕见等位基因。片段长度分布于603～1115bP之间，基因频率分布于0.5～33．5％间，杂合度为64％，DP值为84．5％。对5个家庭25名相关个体进行分析，符合孟德尔遗传定律；对同一个体不同组织的DNA进行P33.4位点的分型研究表明，该技术适宜于法医物证检验。此外，本研究以Chelex处理不同检材制备DNA模板用于扩增，率先建立了比常规方法更快、更简便、更为实用的检验方法。
The amplified fragment length polymorphisms (Amp-FLPs) of p33.4 locus in 100 unrelated individuals in China were studied by polymerase chain reaction (PCR), polyacrylamide gel electrophoresis and silver staining , Detected eight alleles. Data processing via BIOTRAC system. The number of repeat units for each allele was 7, 10 to 15, respectively, with rare alleles having a difference of less than one repeat unit length found between 13 and 14. The fragment length ranged from 603 bp to 1115 bp, the gene frequency ranged from 0.5% to 33.5%, the heterozygosity was 64% and the DP value was 84.5%. The analysis of 25 related individuals in 5 families accorded with Mendel ’s law of inheritance. P33.4 genotyping of DNA from different tissues in the same individual showed that this technique was suitable for forensic evidence test. In addition, Chelex was used to process DNA samples from different samples for amplification. Chelex pioneered a faster, simpler and more practical assay than the conventional methods.