论文部分内容阅读
目的:探讨Yes相关蛋白(YAP)与卵巢癌腹膜及淋巴结转移的相关性。方法:选择148例原发性卵巢癌和30例正常卵巢石蜡切片标本,采用Western blot、免疫组化分析YAP蛋白在正常卵巢组织、非转移性卵巢癌组织及转移性卵巢癌组织中的表达,分析YAP蛋白在卵巢癌组织中的表达与临床病理特征的关系,并应用单因素及多因素logistics分析卵巢癌腹膜和淋巴结转移的独立危险因素。结果:YAP蛋白在正常卵巢组织、非转移性卵巢癌组织及转移性卵巢癌组织中的表达量依次增高,两两比较差异均有统计学差异(P<0.05)。YAP蛋白核过表达与组织学分化、残余病灶、复发、腹膜转移以及淋巴结转移均显著相关(P<0.05)。YAP蛋白核过表达是独立的影响卵巢癌腹膜(OR:5.443;95%CI:2.287-12.950;P<0.001)和淋巴结转移(OR:4.477;95%CI:2.059-9.735;P<0.001)的独立危险因素。结论:YAP基因核过表达与卵巢癌腹膜转移及淋巴结转移密切相关,有可能作为临床上预测卵巢癌腹膜及淋巴结转移的新的分子标记物。
Objective: To investigate the relationship between Yes-related protein (YAP) and peritoneal and lymph node metastasis in ovarian cancer. Methods: 148 cases of primary ovarian cancer and 30 cases of normal ovarian paraffin sections were selected. The expression of YAP protein in normal ovarian tissue, non-metastatic ovarian cancer and metastatic ovarian cancer was analyzed by Western blot and immunohistochemistry. The relationship between the expression of YAP protein and clinicopathological features in ovarian cancer was analyzed. Independent risk factors of peritoneal and lymph node metastasis of ovarian cancer were analyzed by single factor and multi-factor logistics. Results: The expression levels of YAP protein in normal ovarian tissue, non-metastatic ovarian cancer and metastatic ovarian cancer successively increased, with significant difference (P <0.05). YAP protein nuclear translocation was significantly associated with histological differentiation, residual lesions, recurrence, peritoneal metastasis and lymph node metastasis (P <0.05). Overexpression of YAP protein independently affected the peritoneum (OR: 5.443; 95% CI: 2.287-12.950; P <0.001) and lymph node metastasis (OR: 4.477; 95% CI: 2.059-9.735; P <0.001) Independent risk factors. Conclusion: The overexpression of YAP gene is closely related to peritoneal metastasis and lymph node metastasis of ovarian cancer, which may be used as a new molecular marker for clinical prediction of peritoneal and lymph node metastasis of ovarian cancer.