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为筛选板栗混合花序中雌雄花形成与发育关键基因,基于板栗雌雄花表达谱测序结果,从差异表达基因中分离到17个MADS-box基因。通过序列结构分析,发现其中2个基因缺少半保守的K盒,为Ⅰ型MADS-box基因,另外15个基因为Ⅱ型MADS-box基因。系统进化关系分析显示,13个包含完整读码框的MADS-box基因中有2个B类基因,1个C/D类基因和3个E类基因。荧光定量PCR结果证实这些MADS-box基因在板栗早期混合花序中的雌雄花之间表达差异显著(变化倍数>2),且部分B、C/D和E类基因在板栗幼叶、纯雄花和雌花中的表达变化趋势相似,推测这些MADS-box基因在板栗雌雄花形成和发育中起重要调控作用。
To screen for the key genes for the formation and development of male and female flowers in chestnut mixed inflorescences, 17 MADS-box genes were isolated from differentially expressed genes based on the sequencing results of male and female flower expression profiles of chestnut. Sequence analysis showed that two of them lacked a semi-conserved K-box, a type I MADS-box gene and the other 15 were type II MADS-box genes. Phylogenetic analysis showed that there are two B-type genes, one C / D-type gene and three E-type genes in the 13 MADS-box genes containing the complete reading frame. Fluorescence quantitative PCR results confirmed that the expression of these MADS-box genes was significantly different between male and female flowers in mixed chestnut chestnut (> 2 fold change), and some B, C / D and E genes were expressed in young chestnut, The expression changes in female flowers are similar, suggesting that these MADS-box genes play an important regulatory role in the formation and development of male and female flowers.