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AIM:To determine the changes of CD8+ T subsets especiallyCD8+CD28-T regulatory cells in rat model of experimentalcolitis induced by 2,4-dinitrofluorobenzene (DNFB).METHODS:The rat model of experimental colitis wasinduced by enema with DNFB.Ten days later,colonicintraepithelial and splenic lymphooltes were isolated fromcolitis animals (n=16) and controls (n=8).The proportionof CD8+ T cells,CD8+CD28+ T cells and CD8+CD28-Tregulatory cells were determined by flow cytometry.RESULTS:The model of experimental colitis was successfullyestablished by DNFB that was demonstrated by bloodydiarrhea,weight loss and colonic histopathology.The proportionof CD8+ T cells in either splenic or colonic intraepitheliallymphocytes was not significantly different between colitisanimals and controls (spleen:34.6±7.24 % vs 33.5±9.41%,colon:14.0±8.93 % vs 18.0±4.06 %,P>0.05).But CD8+CD28-T regulatory cells from colitis animals were significantly morethan those from controls (spleen:11.3±2.26 % vs 5.64±1.01%,colon:6.50±5.37 % vs 1.07±0.65 %,P<0.05).In contrast,CD8+CD28+ T cells from colitis animals were less than thosefrom controls (spleen:23.3±6.14 % vs 27.8±9.70 %,P=0.06;colon:7.52±4.18 % vs 16.9±4.07 %,P<0.05).The proportionof CD8+CD28-T regulatory cells in splenic and colonintraepithelial CD8+ T cells from colitis animals was higherthan that from controls (spleen:33.3±5.49 % vs 18.4±7.26 %,colon:46.0±14.3 % vs6.10±3.72 %,P<0.005).CONCLUSION:Experimental colitis of rats can be inducedby DNFB with simplicity and good reproducibility.Theproportion of CD8+CD28-T regulatory cells in rats withexperimental colitis is increased,which may be associatedwith the pathogenesis of colitis.
AIM: To determine the changes of CD8 + T subsets especially CD8 + CD28-T regulatory cells in rat model of experimentalcolitis induced by 2,4-dinitrofluorobenzene (DNFB) .METHODS: The rat model of experimental colitis was induced by enema with DNFB.Ten days later , proportionate CD8 + T cells, CD8 + CD28 + T cells and CD8 + CD28-Tregulatory cells were determined by flow cytometry .RESULTS: The model of experimental colitis was successfully established by DNFB that was demonstrated by bloody diarrhea, weight loss and colonic histopathology. proportion of CD8 + T cells in either splenic or colonic intraepitheliallymphocytes was not significantly different between colitisanimals and controls (spleen: 34.6 ± 7.24% vs 33.5 ± 9.41% , colon: 14.0 ± 8.93% vs 18.0 ± 4.06%, P> 0.05) .But CD8 + CD28-T regulatory cells from colitis animals were significantly more than those from controls (spleen: 11.3 ± 2.26% vs 5.64 ± 1.01 %, colon: 6.50 ± 5.37% vs 1.07 ± 0.65%, P <0.05) .In contrast, CD8 + CD28 + T cells from colitis animals were less than those from controls (spleen: 23.3 ± 6.14% vs 27.8 ± 9.70%, P = 0.06; colon: 7.52 ± 4.18% vs 16.9 ± 4.07%, P <0.05). The proportion of CD8 + CD28- T regulatory cells in splenic and colonintraepithelial CD8 + T cells from colitis animals was higherthan that from controls (spleen: 33.3 ± 5.49% vs 18.4 ± 7.26%, colon: 46.0 ± 14.3% vs6.10 ± 3.72%, P <0.005) .CONCLUSION: Experimental colitis of rats can be induced by DNFB with simplicity and good reproducibility.Theproportion of CD8 + CD28-T regulatory cells in rats withexperimental colitis is increased, which may be associated with the pathogenesis of colitis.