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目的观察小檗碱(Berberine,Ber)对实验性大鼠结肠癌的防治作用及其与过氧化物增殖物激活受体γ(Peroxi-some proliferator-activated receptorγ,PPARγ)表达的相关性。方法以二甲肼(1-2 dimethylhydrazine,DMH)40 mg/kg皮下注射加1%葡聚糖硫酸钠(Dextran sodium sulfate,DSS)水溶液饮用诱导形成大鼠结肠癌模型,以罗格列酮(Rosiglitazone,Ros)为阳性对照,观察Ber灌服对大鼠体重、异常隐窝灶(Abrrant crypt foci,ACF)和结肠癌发生率的影响。采用MTT法检测不同浓度Ber对人结肠癌lovo细胞增殖活性的影响;RT-PCR法检测Ber对lovo细胞PPARγ基因mRNA表达的情况。结果 Ber能明显改善结肠癌大鼠的恶液质状态,并阻遏体重减轻,显著减少大鼠结肠ACF数和降低结肠癌的发生率,其作用与Ros相似。Ber可呈浓度和时间依赖性抑制lovo细胞增殖,并降低lovo细胞中PPARγ基因mRNA的表达水平。结论 Ber能抑制大鼠早期ACF的形成和结肠癌发生,其作用机制可能与抑制PPARγ表达有关。
Objective To investigate the preventive and therapeutic effects of Berberine (Ber) on colon cancer in rats and its correlation with the expression of peroxisome proliferator-activated receptor γ (PPARγ). Methods Rat colon cancer model was induced by subcutaneous injection of 1 mg / kg dimethylhydrazine (DMH) 40 mg / kg plus 1% Dextran sodium sulfate (DSS) solution. Rosiglitazone Rosiglitazone, Ros) as positive control to observe the effect of Ber on the body weight, Abrrant crypt foci (ACF) and the incidence of colon cancer in rats. The effects of different concentrations of Ber on the proliferation of human LoVo cells were detected by MTT assay. The mRNA expression of PPARγ gene was detected by RT-PCR. Results Ber can significantly improve the state of cachexia in rats with colon cancer and restrain weight loss, significantly reduce the number of colon ACF and reduce the incidence of colon cancer, the role of Ros and similar. Ber inhibited the proliferation of lovo cells in a concentration- and time-dependent manner and decreased the expression of PPARγ mRNA in lovo cells. Conclusion Ber can inhibit the formation of early ACF and colon cancer in rats. The mechanism may be related to the inhibition of PPARγ expression.