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目的:探讨PTP4A1基因干扰对人舌鳞癌CAL27细胞增殖、凋亡及体外迁移的影响。方法:采用PTP4A1干扰慢病毒转染人舌鳞癌CAL27细胞,实时定量PCR和Western blotting验证PTP4A1干扰效果,分别采用MTT法、划痕试验检测细胞增殖、迁移情况,流式细胞术分析细胞凋亡,Western blotting分析caspase-3、Bax、Bcl-2蛋白表达。结果:PTP4A1干扰慢病毒转染后,干扰组中CAL27细胞PTP4A1基因mRNA和蛋白表达均明显减少。与对照组比较,PTP4A1干扰后,细胞增殖、体外迁移能力均显著减弱(P<0.05);PTP4A1干扰后CAL27细胞凋亡率相比对照组显著增大(P<0.05),PTP4A1干扰促进CAL27细胞凋亡与上调caspase-3、Bax蛋白表达,下调Bcl-2蛋白表达水平有关。结论:PTP4A1基因干扰可抑制人舌鳞癌CAL27细胞增殖活性和体外迁移能力,且促进舌鳞癌细胞凋亡。
Objective: To investigate the effects of PTP4A1 gene silencing on proliferation, apoptosis and migration of human tongue squamous cell carcinoma CAL27 cells in vitro. Methods: Human tongue squamous cell carcinoma cell line CAL27 was transfected with PTP4A1 by lentivirus, and the effect of PTP4A1 was detected by real-time PCR and Western blotting. Cell proliferation and migration were detected by MTT assay and scratch assay respectively. Cell apoptosis was analyzed by flow cytometry Western blotting analysis of caspase-3, Bax, Bcl-2 protein expression. Results: PTP4A1 interference lentiviral transfection, the interference group CAL27 cell PTP4A1 mRNA and protein expression were significantly reduced. Compared with control group, PTP4A1 interference significantly decreased cell proliferation and migration in vitro (P <0.05). The apoptosis rate of CAL27 cells increased significantly after PTP4A1 interference compared with control group (P <0.05), and PTP4A1 interference promoted CAL27 cells Apoptosis is related to up-regulation of caspase-3, Bax protein expression and down-regulation of Bcl-2 protein expression. Conclusion: PTP4A1 gene knockdown can inhibit human tongue squamous cell carcinoma CAL27 cell proliferation and migration in vitro, and promote tongue squamous cell carcinoma apoptosis.