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本研究观察了棉铃疫病病菌(phytophthoraboehmeriae)的侵染前行为及其侵染过程,表明温度和淋溶物影响孢子囊的萌发方式,18~20℃条件下全行间接萌发,22~24℃条件下表现孢子囊直接萌发量增多,并迅速产生次级孢子囊。人工游动孢子接种证明该病菌主要为害棉铃,此外还能有效地侵染棉苗及成株叶片。在铃表在附着胞形成较棉叶上迅速,且有次级乃至三级附着胞产生。棉铃气孔是病菌入侵的主要途径;在叶表该病菌气孔保卫细胞与表皮细胞毗邻的垂周壁侵染。接种体浓度愈高,游动孢子侵染棉叶的潜育期愈短,24小时即能检出新生孢子囊,孢囊梗由气孔伸出;与此同时,在病组织中有性器官也开始形成。在棉病铃壳、内果皮、铃纤维以及种皮中均见有卵孢子。田间遗留的棉花枝叶有富集菌源作用。
In this study, the behavior and infection process of phytophthora boehmeriae before infection were observed. The results showed that the temperature and leaching matter affected the germination mode of sporangium. Indirect germination occurred at 18 ~ 20 ℃, and the condition of 22 ~ 24 ℃ Under the performance of sporangia increased the amount of direct germination, and the rapid generation of secondary sporangia. Artificial zoospore vaccination proves that the bacteria mainly harm cotton bolls, in addition can effectively infect cotton seedlings and adult leaves. In the boll watchticks in the attachment cells formed on the cotton leaf faster, and there are secondary or even three attachment cells. Stomatal is the main path of bacterial invasion; in the leaf surface of the stomatal guard cells and epidermal cells adjacent to the vertical wall infection. The higher the concentration of inoculum, the shorter the incubation period of zoospore infection of cotton leaves, 24 hours to detect neoplasmic sporangia, cyst stomatal extension stick; at the same time, in the diseased tissue also began sexual organs form. In cotton disease bell shell, endocarp, bell fiber and seed coat are seen oospores. Cotton leaves left in the field have enrichment of bacterial sources.